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分光光度法测定植酸含量 被引量:7

Determination on Phytic acid by Spectrophotometry
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摘要 [目的] 为了寻找适合于标准植酸的测定及微量测定的方法。[方法] 采用可见分光光度法, 测定植酸的含量。[结果] 在标准曲线下,植酸浓度在0~32 μg/ml范围内与吸光度呈良好线性关系,方程为:Y=-2.284 2X+0.384 1(R=0.997 3),平均回收率为97.35%,RSD为4.82%(n=4)。[结论]该方法操作简便、快速、准确,可作为植酸的定量分析方法。 [ Objective] The research aimed to find the method suited to the measurement of standard phytic acid and microdetermination. [ Method] A method for determination of phytic acid by speetrophotometry was studied. [ Result] The regression equation within the range of' the standard sample concentration in 0 -32 μg/ml was Y = -2. 284 2X + 0. 384 1 with a good linearity (R = 0. 997 3 ). The average recovery rate was 97.35 % and RSD was 4.82% (n = 4). [ Conclusion] The method was simple, rapid, accurate and suitable for the quality evaluation of phytic acid.
出处 《安徽农业科学》 CAS 2014年第7期1907-1907,1975,共2页 Journal of Anhui Agricultural Sciences
关键词 植酸 含量 可见分光光度法 Phytic acid Content Spectrophotometry
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