摘要
建立了整体柱固相萃取-高效液相色谱/紫外检测在线联用方法并用于水果中氯吡脲的分析测定。考察了萃取溶剂的 pH 值及其流速、溶剂解吸时间及其流速等因素对氯吡脲萃取率的影响。在优化的条件下，该方法对氯吡脲的富集倍数为214倍，方法的线性范围为0.01-50.00μg / L，检出限为25 ng / L，相对标准偏差（ RSD）为3.9％。黑葡萄、猕猴桃和西瓜等水果中氯吡脲的加标回收率为87.0％-120.7％，RSD 为0.6％-9.2％。结果表明该方法快速、灵敏，能满足水果中痕量氯吡脲残留分析的要求。
A novel method based on the monolithic column solid-phase extraction（SPE）cou-pled with high performance liquid chromatography in online mode was developed for the deter-mination of trace forchlorfenuron in fruits. The enrichment factor of SPE for forchlorfenuron was 214. The method was fully validated. It showed a linear range of 0. 01-50. 00 μg / L and a limit of detection of 25 ng / L,with the relative standard deviation（RSD）of 3. 9% . The proposed method was successfully applied for the determination of forchlorfenuron in grape,kiwifruit and watermelon samples,with the recoveries of 87. 0% -120. 7% and the RSDs of 0. 6% -9. 2% . This online method was proved to be selective,sensitive and convenient for the determination of trace forchlorfenuron in complex samples.
出处
《色谱》
CAS
CSCD
北大核心
2014年第4期407-412,共6页
Chinese Journal of Chromatography
基金
国家自然科学基金项目(21127008)
高等学校博士学科点专项(20120171110001)
广州市科技计划项目(12A102051514)
关键词
整体柱
固相萃取
高效液相色谱
在线分析
氯吡脲
水果
monolith column
solid-phase extraction
high performance liquid chromatography（ HPLC ）
online analysis
forchlorfenuron
fruits
