摘要
建立了高效液相色谱法同时测定复方金霉素膜中的盐酸金霉素、盐酸达克罗宁和醋酸泼尼松.采用Diamonsil C18色谱柱,以乙腈：0.2％磷酸为流动相,梯度洗脱,检测波长254 nm.盐酸金霉素、盐酸达克罗宁和醋酸泼尼松分别在40.0～120.0、15.0～45.0和5.0～15.0 μg/ml浓度范围内线性关系良好.三者的平均加样回收率分别为101.0％、100.1％和100.3％,RSD为0.91％、1.3％和1.1％.
An HPLC method was established for the determination of chlortetracycline hydrochloride, dyclonine hydrochloride and prednisone acetate in compound chlortetracycline film. A Diamonsil C18 column was used, with the mobile phase of acetonitrile ： 0.2 % phosphoric acid by gradient elution at the detection wavelength of 254 nm. The calibration curves for chlortetracycline hydrochloride, dyclonine hydrochloride and prednisone acetate were linear in the ranges of 40.0 - 120.0, 15.0 - 45.0 and 5.0 - 15.0 ug/ml, respectively. Their average recoveries were 101.0 %, 100.1% and 100.3 %, with RSDs of 0.91%, 1.3 % and 1.1%, respectively.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
2014年第4期370-373,共4页
Chinese Journal of Pharmaceuticals
基金
军队医疗机构制剂标准提高计划(13ZJZ08)
关键词
盐酸金霉素
盐酸达克罗宁
醋酸泼尼松
复方金霉素膜
高效液相色谱
测定
chlortetracycline hydrochloride
dyclonine hydrochloride
prednisone acetate
compound chlortetracycline film
HPLC
determination
