摘要
为探讨谷氨酰胺(Gln)对嗜水气单胞菌致病中华鳖的保护作用,本试验选取健康中华鳖270只,随机分为5组(A^E组),每组3个重复,每个重复18只。A^D组腹腔注射嗜水气单胞菌(1.3×109cfu/mL,0.5 mL),E组注射等量生理盐水。6 d后,A、E组注射生理盐水,B、C、D组分别注射100 mg/kg·BW、200 mg/kg·BW、400 mg/kg·BW Gln注射液,隔天注射,共注射3次。观测各组中华鳖的存活率,血浆总抗氧化能力(T-AOC)、谷胱甘肽过氧化物酶(GSH-PX)、丙二醛(MDA)、溶菌酶(LZM),脾脏、肝脏和肠道的组织学以及肠道菌群变化。结果表明,A组血浆LZM极显著低于C、D、E组(P<0.01),C、D组LZM含量极显著高于A组(P<0.01);A组T-AOC极显著低于E组(P<0.01),D组T-AOC极显著高于A、B、C组(P<0.01);A组GSH-PX极显著低于其余各组(P<0.01);A组MDA极显著高于E组(P<0.01),B、C、D组MDA显著低于A组(P<0.05);A组中华鳖脾脏的红髓脾窦淤血扩张,红髓和白髓的淋巴细胞和血细胞坏死。肝脏充血严重,大量肝细胞破裂,肝细胞间有大量红细胞浸润。中肠黏膜萎缩,较多皱襞断裂。随着Gln注射剂量的增加,B、C、D组脾脏红细胞充斥逐渐减少,红白髓的分界逐渐明显。肝脏出血点减少,肝细胞的大小与排布也渐趋正常。中肠皱襞破损脱落程度逐渐减轻;A组肠道嗜水气单胞菌和大肠杆菌明显高于E组(P<0.05),而乳酸杆菌和芽孢杆菌明显低于E组(P<0.05);D组嗜水气单胞菌和大肠杆菌明显低于A组(P<0.05),而乳酸杆菌和芽孢杆菌明显高于A组(P<0.05)。注射Gln可缓解嗜水气单胞菌对中华鳖的致病作用,有效恢复机体抗氧化及非特异免疫力,降低死亡率。
This study investigated the protective effect of Gln on Pelodiscus sinensis against Aeromonas hydrophila infection. A total of 270 healthy Pelodiscus sinensis were randomly divided into 5 groups. Groups A, B, C, D were injected with Aeromonas hydrophila （1.3×10^9 cfu/mL,0.5 mL） and group E was injected with equivalent normal saline as control. Groups A and E were injected with normal saline, and 100, 200 and 400 mg/kg glutamine were designed to inject the other three groups after the pathological symptoms appeared （after 6 d infection）. Then the survival rate, T-AOC, GSH-PX, MDA and LZM values in plasma, histological changes including liver, spleen and intestine, and the change of the intestinal flora of each group was recorded. The result showed that the LZM and T-AOC in plasma of group A was significantly decreased compared with groups C, D and E （P〈0.01）; The A-TOC value of group A was significantly decreased compared with groups D and E （P〈0.01）, furthermore, the value s of groups B and C were significantly lower than that of group D （P〈0.01）; The GSH-PX of group A was significantly lower than other groups （P〈0.01）; The MDA of group A was significantly higher than that of other groups （P〈0.05）. The red pulps of spleen sinusoids were congested, and necrosis of most lymphocytes and blood cells was observed in the red and white pulp; the cells of liver was broken and infiltrated with red cells, and abiogenetic desquamation of the plasma was observed in intestine of group A. Along with the increasing doze of Gln, the number of red blood cells of spleen was decreased and the boundary line of pith became clear; the liver bleeding location was reduced, the number of liver cell and midgut plica were back to normal in groups B, C and D; The Aeromonas hydrophila and E. coli in intestinal of group A was significantly higher than group E （P〈0.05）, while bacillus and lactic was significantly lower than group E （P〈0.05）. The Aeromonas hydrophila and E. coli in intestinal of group A was significantly higher than group D （P 〈 0.05）, while bacillus and lactic was significantly lower than group D （P〈0.05）. In conclusion, Gln play a vital role in alleviating Pelodiscus sinensis agaisnt Aeromonas hydrophila infection.
出处
《四川动物》
CSCD
北大核心
2014年第2期254-260,共7页
Sichuan Journal of Zoology
基金
四川省教育厅资助项目(11ZA292)
关键词
中华鳖
嗜水气单胞菌
谷氨酰胺
Pelodiscus sinensis
Aeromonas hydrophila
glutamine
