摘要
目的：建立了芡实中不同构型维生素E的RP-HPLC含量测定方法。方法：采用高效液相色谱分析,Waters XBridge C18柱（4.6mm×150mm,5μm）,以A相甲醇和B相水（97∶3）为流动相等度洗脱,流速为1.0mL/min,柱温：30℃,检测波长为295nm。结果：α-生育酚、β＋γ-生育酚、δ-生育酚均能够达到基线分离,α-生育酚在0.12~1.2g/L范围内具有良好的线性关系,R21=0.9998;β＋γ-生育酚在0.056~0.56g/L范围内具有良好的线性关系,R22=0.9999;δ-生育酚则在0.0096~0.096g/L范围内具有良好的线性关系,R23=0.9998。平均加样回收率分别为100.47%,100.04%,99.10%;RSD分别为0.27%,4.65%,1.92%。安徽安庆芡实中总VE含量最高,可达2.2633mg/g。结论：该方法简便、准确、重现性好,为芡实药材的质量评价提供合理的参考依据。
Objective ： A reversed- phase high performance liquid chromatographic method（ RP- HPLC） was developed for determination of different configuration of vitamin E in Euryale Semen. Methods：RP-HPLC determination was performed on a Waters Xbridge C18column（4.6mm×150mm,5μm）,Methanol-water（97∶3）was used as the mobile phase at the flow rate of 1.00mL/min,the column temperature was kept as 30℃ and the detection wavelength was set as 295nm. Results ： α-tocopherol, β ＋γ-tocopherol, δ-tocopherol were separated in baseline,the linear range was 0.12~1.2g/L for α-tocopherol,0.056~0.56g/L for β＋γ-tocopherol and 0.0096~0.096g/L for δ-tocopherol. R2were 0.9998,0.9999,0.9998,the average recoveries were 100.47%,100.04%,99.10%. RSD were 0.27%,4.65%,1.92%. The total Vitamin E content of the sample of Anhui was the highest with 2.2633mg/g. Conclusion：This method was sample,accurate and with good reproducibility,which could be used as Euryale Semen quality control of medicine and also provides a scientific reference for the rational use of Euryale Semen.
出处
《食品工业科技》
CAS
CSCD
北大核心
2014年第8期74-78,共5页
Science and Technology of Food Industry
基金
"十二五"国家科技支撑计划项目(2011BAI04B06)
江苏省优秀科技创新团队"中药资源化学"(2011)
