摘要
建立测定调味品中7种工业合成染料的高效液相色谱法。样品经乙腈超声提取后，10000r／min离心10min，取上清液进行分析，以0．02mol／乙酸铵溶液和乙腈溶液为流动相进行梯度洗脱，二极管阵列检测器测定。结果表明：7种工业合成染料的分离效果良好，回收率为86．8％～106．0％，相对标准偏差为1．07％-5．44％（n=5）；检出限为0．2-0．5mg／kg。该方法操作简单快速，结果准确，重复性好，适用于调味品中7种工业合成染料的同时测定。
An HPLC method for the determination of seven industrial synthetic dyes in condiments was established. Samples were extracted with acetonitrile under ultrasonic-assisted conditions and the extract was centrifuged. The supernatant was then analyzed by HPLC-DAD with gradient elution using a mobile phase consisting of 0.02 mol/L ammonium acetate and acetonitrile and quantified by an external standard method. The concentrations of seven industrial synthetic dyes showed good linearity with peak area, and good separation was achieved for them. Their recovery rates from a spiked sample were in the range of 86.8%-106.0%, and the relative standard deviations were 1.07%-5.44% （n = 5）. The detection limits were in the range of 0.2-0.5 mg/kg. In conclusion, this method is rapid, simple, sensitive, accurate and reproducible, and has a good applicability to the simultaneous determination of seven industrial synthetic dyes in condiment products.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2014年第6期168-171,共4页
Food Science
关键词
反相高效液相色谱
工业合成染料
调味品
high performance liquid chromatography
condiment
industrial synthetic dyes
