摘要
以蓝莓为原料,在单因素试验基础上,以乙醇体积分数、液料比、提取时间、pH和提取温度为响应因素,花青素提取量为响应值,采用5因素3水平的响应面分析法,确定最佳提取工艺条件为:提取温度50℃、料液比1∶20(g/mL)、乙醇体积分数70%、pH 2、提取时间1 h,提取量为0.444 g/L。经测定,蓝莓花青素具有较强的DPPH自由基和ABTS自由基清除能力及还原能力,可作为优良的天然抗氧化剂来源。
On the basis of single-factor experiments, the optimal extraction conditions for maximizing blueberry anthocyanin were determined by using a 5-variable, 3-level Box-Behnken experimental design combined with response surface analysis as follows： the material-liquid ratio was 1 ： 20 （g/mL）; the extraction temperature was 50 ℃; the extraction time was 1 h; the ethanol concenWation was 70%, resulting in an blueberry anthocyanin yield of 0.444 g/L. At the same time, Blueberry anthocyanin was found to have powerful DPPH free radical, ABTS free radical scavenging capacity and reducing power. It could be developed as excellent natural antioxidant resources.
出处
《食品工业》
北大核心
2014年第4期39-44,共6页
The Food Industry
基金
国家级大学生创新创业训练计划项目(201210635031)
关键词
蓝莓
花青素
响应曲面法
抗氧化活性
blueberry
anthocyanin
response surface methodology
antioxidant activity
