摘要
[目的]应用组织培养技术对紫色马铃薯进行离体培养芽诱导研究,以建立紫色马铃薯离体培养技术体系。[方法]以紫色马铃薯芽为外植体,进行不同的灭菌处理、基本培养基、蔗糖浓度和培养温度的比较试验,筛选紫色马铃薯芽诱导的适宜因子。[结果]试验结果表明:适宜的芽外植体灭菌处理为75%酒精10 s+0.1%升汞10 min,芽诱导成活率为45.56%;适宜的基本培养基是MS培养基,芽诱导成活率为48.89%;适宜的蔗糖浓度是30g/L,芽诱导成活率为58.89%;适宜的培养温度是25℃,芽诱导成活率为54.44%。[结论]研究结果为紫色马铃薯快速繁殖提供了技术参考。
[Objective] The aim was to use tissue culture technology for bud induction in vitro culture, in order to establish the technique system in vitro culture of purple potato. [Method] Using bud of purple potato as the explant, making compared experiments on different sterilization treatments, basic media, sucrose concentration and culture temperature, to select the suitable factors on the bud induction of purple potato. [Result] The experimental results indicated that the suitable treatment on sterilization of bud explant was 75%Ethanol （10s）＋0.1%HgCl2 （10min） and the survival rate of bud induction showed 45.56%,the suitable basic medium was MS and the rate showed 48.89%,the suitable sucrose concentration was 30g/L and the rate showed 58.89% and the suitable culture temperature was 25℃ and rate showed 54.44%. [Conclusion] The results provided technical references for potato rapid propagation.
出处
《园艺与种苗》
CAS
2014年第1期23-25,28,共4页
Horticulture & Seed
关键词
紫色马铃薯
芽外植体
离体培养
诱导因子
培养条件
Purple potato
Bud explant
Culture in vitro
Induction factor
Cultural condition
