摘要
目的探讨人参皂苷Rg3对H2O2所致海马神经元损伤的保护作用及其可能机制。方法人参皂苷Rg3预处理体外培养C57小鼠胎鼠海马神经元,再加入H2O2继续培养,显微镜观察细胞状态并采用细胞活性检测(CCK-8)神经元的活性,生化法测定乳酸脱氢酶(LDH)释放量和丙二醛(MDA)、超氧化物歧化酶(SOD)的活性,实时荧光定量聚合酶链式反应(RT-PCR)检测神经元凋亡相关基因Caspase-3 mRNA的表达。结果与模型组比较,中、高浓度人参皂苷Rg3预处理组细胞状态好于模型组,细胞活性明显增强,培养液中LDH的漏出量明显降低,细胞内MDA的生成明显减少,SOD活性增强,Caspase-3 mRNA表达显著下调(P<0.05)。结论人参皂苷Rg3预处理对H2O2所致海马神经元的损伤具有保护作用。
AIM To investigate the protective effect of ginsenoside Rg3 on hippocampal neuron. METHODS Hippocampal neuron was treated with ginsenoside Rg3 （2, 10, 50 μmol/L） for 30 min prior to H202 （50. 0 μmol/L） treatment. After 6 h, neuron morphology was observed by microscope, cell viability was tested with CCK-8, LDH, MDA level and SOD viability were measured by biochemical methods, caspase-3 mRNA ex pression was measured by RT-PCR. RESULTS Compared to the control group, neuron and SOD viability de- creased （P 〈0. 05） while LDH, MDA and Caspase-3 mRNA level increased （P 〈 0.05 ） in the model group, groups pretreated with ginsenoside Rg3 （10, 50 μmol/L）, neuron and SOD viability increased （P 〈 0.05）, while LDH, MDA and Caspase-3 mRNA level decreased （P 〈 0.05 ）. CONCLUSION Ginsenoside Rg3 can protect H202 induced hippocampal neuron toxicity maybe through decreasing LDH, MDA and caspase-3 mRNA lev el and increasing SOD viability.
出处
《中成药》
CAS
CSCD
北大核心
2014年第4期670-674,共5页
Chinese Traditional Patent Medicine
基金
云南省高校重点实验室基金项目(2011YXZD07)
