摘要
为比较不同品种羊的重组干扰素刺激基因15（IFN—stimulated gene，ISG15）蛋白对淋巴细胞转化效果影响的差异，试验比较了5种浓度的盘羊、巴什拜羊及其杂交羊重组ISG15蛋白对培养的绵羊外周血淋巴细胞转化的影响。结果表明，添加盘羊重组ISG15蛋白浓度为10-80ug／mL时，D570nm值均显著高于PBS对照组（P〈0．05）；添加巴什拜羊重组ISG15蛋白浓度为40～80ug／mL时，D570nm值均显著高于PBS对照组（P〈0．05）；而各种浓度的杂交羊ISG15组与PBS对照组相比差异均不显著（P〉0．05）。提示，盘羊和巴什拜羊的重组ISG15蛋白在一定浓度下能有效地刺激淋巴细胞转化，且随蛋白浓度的增加对淋巴细胞转化作用增强，而杂交羊重组ISG15蛋白对外周血淋巴细胞转化无显著影响。
In order to compare the different effects of recombinant ISG15 proteins in different varieties of sheep on lymphocyte transformation,we compared the effects of five different concentrations recombinant ISG15 proteins of Argali, Bashibai sheep and their hybrid sheep on the sheep peripheral blood lymphocyte transformation. The results showed that when the recombinant ISG15 protein concentrations of Argali sheep were from 10 to 80 ug/mL,D570 nm were significantly higher than PBS control group （P〈0.05）; the recombinant ISG15 protein concentrations of Basbibai sheep were from 40 to 80 ug/mL,D570 nm were significantly higher than PBS control group （P〈0.05） ; while the recombinant ISG15 protein of hybrid sheep, compared with PBS control group, had no significant difference （P〈 0.05）. In conclusion, certain concentrations of the recombinant ISG15 proteins of Argali and Bashibai sheep could stimulate lymphocyte transformation,with the increase of protein concentrations, the effects of lymphocyte transformation were enhanced,while the recombinant ISG15 protein of hybrid sheep had no significant effect on peripheral blood lymphocyte transformation.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第4期153-155,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金"类泛素ISG15在盘羊
巴什拜羊及其杂交种的疾病抗性研究"(31060351)
关键词
绵羊
干扰素刺激基因15
淋巴细胞
转化
sheep
interferon-stimulated gene 15
lymphocytes
transformation
