摘要
用紫外光谱、荧光光谱、圆二色谱法等方法研究了抗癌药物５－氟尿嘧啶（５－ｆｌｕｏｒｏｕｒａｃｉｌ，５－ＦＵ）与ＤＮＡ、血清蛋白的相互作用及某些二价金属离子对其作用的影响。求得了５－ＦＵ与ＨＳＡ、ＢＳＡ的结合常数。
The interaction of 5-fluorouracil (5-FU) with deoxyribonucleic acid (DNA) and serum albumin and the influence of some metal ions on the interaction have been studied by using spectral methods of UV-absorption, fluorescence and circular dichroism. It was shown that the interaction of 5-FU with DNA and serum albumin was effected by some metal ions. The binding costant of 5-FU with serum albumin was found to be K-HSA = 4.38 x 10(3) (mol/L)(-1) and K-BSA = 4.95 x 10(3) (mol/L)(-1) and the distance between the bound 5-FU and the tryptophan residue mrs also determined to be r(HSA) = 3.53 nm, r(BSA) = 3.51 nm.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2001年第2期192-194,共3页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金!(No.29575200)
天津医科大学自然科学基金资助课题。
