摘要
目的:建立保肝降脂颗粒的质量标准.方法:采用TLC对保肝降脂颗粒中丹参、香附、片姜黄进行定性鉴别;通过HPLC同时测定保肝降脂颗粒中绿原酸和丹酚酸B含量,流动相乙腈(A)-0.5%甲酸水溶液(B)梯度洗脱(0~ 10 min,10%A;10~20 min,10% ~ 15% A;20 ~30 min,15% ~ 20%A;30 ~ 53 min,20% ~ 30%A;53~60 min,30% ~ 10%A),绿原酸、丹酚酸B检测波长分别为327,286 nm.结果:建立的丹参、香附、片姜黄TLC鉴别方法中斑点清晰且阴性对照无干扰.绿原酸、丹酚酸B线性范围分别为0.050 8~0.571 5,0.326 ~ 3.26 μg,平均回收率分别为100.56%(RSD 2.00%),100.50%(RSD1.59%).结论:建立的方法定性专属性强、定量准确度高,适用于保肝降脂颗粒的质量控制.
Objective:To establish quality standards of Baogan Jiangzhi granules.Method:TLC was used to identify Salviae Miltiorrhizae Radix et Rhizoma,Cyperi Rhizoma and Rhizoma Wenyujin Concisum in Baogan Jiangzhi granules.HPLC was adopted to determine contents of chlorogenic acid and salvianolic acid B with mobile phase of acetonitrile (A)-0.5% formic acid (B) gradient elution (0-10 min,10% A; 10-20 min,10%-15%A; 20-30 min,15%-20% A; 30-53 min,20%-30% A; 53-60 min,30% ~ 10% A),detection wavelength of these two ingredients were 327 and 286 nm,respectively.Result:Established TLC had clear spots without interference of the negative control sample.Linear ranges of chlorogenic acid and salvianolic acid B were 0.050 8-0.571 5 and 0.326-3.26 μg,average recoveries were 100.56% (RSD 2.00%) and 100.50% (RSD 1.59%),respectively.Conclusion:These methods was accurate and specific,which were suitable for quality control of Baogan Jiangzhi granules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期44-47,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81001640)
江苏高校优势学科建设工程项目(ysxk-2010)
关键词
保肝降脂颗粒
丹参
香附
片姜黄
绿原酸
丹酚酸B
高效液相色谱法
薄层色谱法
Baogan Jiangzhi granules
Salviae Miltiorrhizae Radix et Rhizoma
Cyperi Rhizoma
Rhizoma Wenyujin Concisum
chlorogenic acid
salvianolic acid B
HPLC
TLC