摘要
目的:探讨单味菟丝子(Cuscutae Semen,CS,醇提+水提总提取物)及其主要成分兔丝子黄酮(Flavones from Cuscutae Semen,FCS)对肾虚排卵障碍模型大鼠的影响。方法:48只雌性SD大鼠随机分为菟丝子高、低剂量组,菟丝子黄酮高、低剂量组,模型组和空白组,以羟基脲(600 mg·kg-1·d-1)灌胃建立肾虚排卵障碍模型,羟基脲灌胃后2 h各组予相应药物灌胃治疗,共计8 d,治疗后比较各组大鼠子宫指数、卵巢指数、血清性激素水平、成熟卵泡比例、黄体数。结果:①与正常组比较,模型组大鼠出现肾虚表现,卵巢病理切片提示排卵障碍,且大鼠血清雌二醇(E2)、卵泡刺激素(FSH)、黄体生成素(LH)水平(3.68±1.18)pmol·L-1,(2.43±0.24)U·L-1,(2.84±1.05)U·L-1明显低下(P<0.05);②与模型组比较,菟丝子高剂量组大鼠子宫指数、卵巢指数、成熟卵泡比例、血清E2,FSH水平(5.49±1.72)pmol·L-1,(2.70±0.22)U·L-1明显增加(P<0.05);菟丝子黄酮低剂量组大鼠子宫指数、卵巢指数、成熟卵泡比例、血清E2、FSH、LH水平(6.25±2.23)pmol·L-1,(2.78±0.14)U·L-1,(3.65±0.78)U·L-1明显增加(P<0.05)。菟丝子黄酮低剂量组血清LH水平(3.65±0.78)U·L-1明显高于菟丝子高剂量组(3.10±0.60)U·L-1(P<0.05)。结论:菟丝子可有效改善羟基脲引起的肾虚排卵障碍,这种影响可能基于其主要成分黄酮的作用。
Objective：This study was to explore the effects of Cuscutae Semen （CS） and its major components,flavones,on kidney deficiency and ovulation inhibition in female rats.Method：Forty eight SD female rats were randomly divided into 6 groups：CS high and low dose groups,flavones from Cuscutae Semen （FCS） high and low dose groups,model group and normal group.Hydroxyurea emulsion （600 mg·kg-1） was administered intragastrically to establish kidney deficiency model with ovulation inhibition.Two hours after administration of hydroxyurea,rats in each group were treated with corresponding drugs for 8 days.A range of parameters were observed,including the uterine index,the ovarian index,the proportion of mature follicles,the number of corpus luteum,the level of serum estrogen （E2）,follicle-stimulating hormone （FSH） and luteinzing hormone （LH）.Result：①The model rats exhibited symptoms of kidney deficiency,with histopathological assay indicating ovulation inhibition,and the level of serum E2,FSH and LH （3.68 ± 1.18） pmol·L-1,（2.43 ± 0.24） U · L-1,（2.84 ± 1.05） U · L-1 decreased significantly compared to those in the normal contrast group （P ＜ 0.05）.②Compared to the model group,the uterine and ovarian index and the proportion of mature follicles,the serum E2 and FSH levels （5.49 ± 1.72） pmol ·L-1,（2.70 ±0.22） U ·L-1 in CS high dose group were much higher,while in FCS low dose group,all parameters were significantly elevated except for the number of corpus luteum.The increases in the groups were statistically significant （P ＜ 0.05）.Serum LH level in FCS low dose group （3.65 ±0.78） U ·L-1 was significantly higher than that in CS high dosage group （3.10 ±0.60） U ·L-1 （P ＜0.05）.Conclusion：CS could effectively improve the ovulation inhibition and renal deficiency induced by the hydroxyurea.The effect of CS on kidney deficiency and ovulation inhibition model may likely be attributed to its main component-flavones,which is a promising component of traditional chinese medicine.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第8期169-172,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省人口和计划生育委员会科研项目(20110272)
关键词
菟丝子
菟丝子黄酮
肾虚排卵障碍
卵巢内分泌功能
Cuscute Senmen
flavones from Cuscutae Semen
kidney deficiency and ovulation inhibition
ovarian endocrine function
