摘要
植物MAPKs(mitogen-activated protein kinases)在多种生物和非生物胁迫中起重要作用。课题组前期克隆了甘蓝型油菜BnMAPK1基因,并获得BnMAPK1超量表达植株。本文以甘蓝型油菜中油821DH系为对照,以超量表达BnMAPK1的转基因油菜为试材,采用离体叶片接种的方法,测定染病叶片病斑的大小及病斑周围叶片的草酸含量,并用qRT-PCR检测转基因植株4个病程相关蛋白(OXO、Cu/Zn-SOD、PR2、PR3)编码基因在核盘菌胁迫下的相对表达动态变化。结果表明,甘蓝型油菜BnMAPK1超量表达可显著抑制核盘菌对离体叶片的侵染,控制染病叶片内草酸毒素的积累,可能可以解除核盘菌对OXO表达的负调控,使另外3个与病程相关蛋白基因(Cu/Zn-SOD、PR2、PR3)表达上调。表明BnMAPK1超量表达可有效提高油菜菌核病抗性。
Plant MAPKs （mitogen-activated protein kinases） play an important role in defense to biotic and abiotic stresses. In our previous studies, BnMAPK1 was cloned in Brassica napus and transgenic oilseed rape plants overexpressing BnMAPK1 were obtained. In this study, B. napus ZY821DH line was used as control and the corresponding transgenic plants overexpressing BnMAPKI were used as experimental materials. The resistance to S. sclerotiorum and oxalic acid content were tested by using detached leaves inoculated with the pathogen. The dynamic changes of the relative expression of four pathogenesis-related genes, OXO, Cu/Zn-SOD, PR2, and PR3 were detected in the leaves inoculated with the pathogen. The results showed that the overexpression of BnMAPK1 significantly inhibited the invasion of the pathogen, controlled the accumulation of oxalic acid in the inoculated leaves, and maybe finally relieved the negative regulation of OXO expression caused by the pathogen and up-regulated the expression of the other three genes, Cu/Zn-SOD, PR2, and PR3. The results indicated that overexpression of BnMAPK1 can effectively improve the resistance to S. sclerotiorum in oilseed rape.
出处
《作物学报》
CAS
CSCD
北大核心
2014年第4期745-750,共6页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31271756
31171619)
高等学校学科创新引智计划(111计划)项目(B12006)资助
