摘要
制备了4，5-二氯邻苯二胺缩3，5-二溴水杨醛席夫碱（L）及其镍配合物（NiL），以4，5-二氯邻苯二胺席夫碱镍配合物一氧化石墨烯（NiL—rGO）为电极修饰剂，制备修饰玻碳电极，研究了L-酪氨酸在修饰电极上的循环伏安行为。对实验条件进行了优化，结果表明支持电解液为PBS（pH=7．0）缓冲溶液，扫描速率为100mV／s和修饰剂悬浮液用量为10μL时氧化峰电流具有最大值；同时研究还显示，当L-酪氨酸浓度为1．0×10-6～1．0×10-3μmol／L范围内时，浓度与峰电流成良好的线性关系，回归方程为Ip（μA）=0．319C（μmol／L）＋0．179，相关系数为0．995（n=5），检出下限为2．3×10-7mol／L（S／N=3），该方法用于检测啤酒中的L-酪氨酸的含量，RSD为1．26％～4．02％，加标回收率为88．11％-98．50％，结果较满意。
This paper synthesizes a 4,5-dichloro-o-phenylenediamin-3,5-the dibromo alkali Nickel complex and a novel electrochemical method was developed for the detection of L-tyrosine based on the 4,5-dichloro-o-phenylenediamine-graphene oxide modified glassy carbon electrode. The experiment conditions are optimized and the results show that the oxidation peak current value reaches the maximum when supporting electrolyte is PBS （pH=7.0） buffer solution and the scanning rate is 100 mV/s and the dosage modification is 10μL. In addition, the density has a good liner relationship with the peak current on condition that the density range of L-tyrosine is between 1.0×10-6～1.0×10-3mol/L and the regression equation is Ip（pA）=0.319C（μmol/L）＋0.179 with a correlation coefficient of 0.995 （n=5） and the detection lower limit is2.3×10-7 mol/L（S/N=3）. Using this method to detect the L-tyrosine content in beers, this paper concludes that the RSD is 1.26%-4.02% and the recovery is 88.11%-98.50% with satisfactory results.
出处
《食品科技》
CAS
北大核心
2014年第4期271-275,共5页
Food Science and Technology
基金
国家自然科学基金项目(21266006)
广西自然科学基金项目(2012GXNSFAA053034)
广西矿冶与环境科学实验中心项目(KH2012YB002)
