摘要
目的：分析不同孕周胎儿脐血血红蛋白的组成,探讨毛细管电泳技术在定量分析中晚孕胎儿脐血血红蛋白中的应用价值.方法：收集在产前诊断中心行B超引导下脐静脉穿刺术,并剔除夫妻双方或一方确诊或可疑为地中海贫血的孕妇脐带血样本,对其进行血红蛋白毛细管电泳分析.结果：全部425例样本HbA含量间于2.3％ ～ 18.0％,平均6.80％;HbF含量间于82.0％～97.7％,平均值93.18％.随着胎儿孕龄的增加,脐静脉血中HbA的百分含量逐渐增多,HbF的百分含量逐渐减少,且各孕龄组之间HbA百分含量及HbF百分含量的差异均具有统计学意义（P＜0.05）.结论：毛细管电泳技术定量分析血红蛋白,简便、准确,可用于中晚孕胎儿脐血血红蛋白电泳,并为诊断胎儿地中海贫血和异常血红蛋白病提供了参考依据.
Objective To explore the applicative value of using capillary electrophoresis in quantitative analysis of hemoglobin in the fetus cord blood during the second and third trimester pregnancy. Method Collected the fetal cord blood from the pregnant women who had eordocentesis under the guide of ultrasound in prenatal diagnosis center,removed the samples from ones who had been diagnosed or suspected thalassemia in one or both partners, then did quantitative analysis for the hemoglobin by capillary electrophoresis. Results Of all 425 cord blood samples,the amount of HbA was from 2.3% to 18.0% and the average was 6. 80% , the HbF was at levels of 82. 0% - 97. 7% and the average was 93. 18%. With the increase of gestational age,the amount of HbA in fetal cord blood gradually increased,and at the same time, the content of HbF decreased. The difference of the content of HbA and HbF in every gestational age group was statistically significant（ P 〈 0. 05 ）. Conclusion Quantitative analysis of hemoglobin by capillary eleetrophoresis is convenient and accurate ,so it can be used for hemoglobin electrophoresis of fetal cord blood during the second and third trimester pregnancy. This investigation can provide reference data for the diagnosis of thalassemia and abnormal hemoglobin disease.
出处
《吉林医学》
CAS
2014年第10期2086-2088,共3页
Jilin Medical Journal
基金
中山市医学科研基金项目[项目编号:J2011082]
关键词
血红蛋白
脐血
毛细管电泳
Hemoglobin
Fetal cord blood
Capillary electrophoresis
