摘要
目的 探讨慢性牙周炎患者外周血淋巴细胞程序性死亡分子1（programmed death-1,PD-1）及其主要配体程序性死亡分子配体1（programmed death ligand-1,PD-L1）的表达及其意义,以阐明其在慢性牙周炎发生和发展中的作用.方法 采用流式细胞术检测30例慢性牙周炎患者（牙周炎组）、25慢性牙龈炎患者（牙龈炎组）及18例牙周健康对照者（健康对照组）外周血CD4＋和CD8＋T淋巴细胞表面PD-1及PD-L1的表达,并检测16例慢性牙周炎患者进行牙周基础治疗6周后外周血CD4＋和CD8＋T淋巴细胞PD-1及PD-L1的表达.两组间比较采用t检验,多组间比较采用方差分析,多个样本间的两两比较采用LSD-t检验或x2检验.结果 牙周炎组外周血CD4＋、CD8＋T淋巴细胞表面PD-1的阳性表达率[（16.7±5.5）％、（20.8±5.1）％]及牙龈炎组二者的表达率[（14.2±6.1）％、（14.5±4.3）％]均显著高于健康对照组[分别为（9.5±2.1）％、（8.1±1.9）％]（P值均＜0.05）;牙周炎组外周血CD4＋、CD8＋T淋巴细胞表面PD-L1的阳性表达率[（24.2±7.1）％、（15.3±6.8）％]及牙龈炎组二者的表达率[（12.4±6.0）％、（11.2±5.5）％]均显著高于健康对照组[（4.7±1.2）％和（3.2±2.3）％]（P值均＜0.05）.牙周基础治疗6周后,16例慢性牙周炎患者CD4＋和CD8＋T淋巴细胞PD-1、PD-L1的阳性表达率均显著降低,与治疗前相比差异均有统计学意义（P值均＜0.05）.结论 慢性牙周炎患者外周血CD4＋和CD8＋T淋巴细胞PD-1及PD-L1表达水平显著升高,与牙周炎症水平密切相关,牙周基础治疗可以下调慢性牙周炎患者外周血CD4＋和CD8＋T细胞表面PD-1及PD-L1的表达.
Objective To investigate the expression of programmed death-1（PD-1） and programmed death ligand-1 （PD-L1） in peripheral T-lymphocytes from patients with chronic periodontitis and its significance and to clarify its role in the development of chronic periodontitis.Methods A total of 73 subjects were included in the study and divided into three groups,chronic periodontitis （30 cases）,chronic gingivitis（25 cases） and 18 healthy controls.The peripheral blood was collected and PD-1/PD-L1 expression in the surface of CD4＋ T lymphocytes and CD8＋ T lymphocytes was examined by flow cytometry.Blood samples from 16 chronic periodontitis patients were collected at week 0 and 6 after initial therapy for 6 weeks and PD-1 and PD-L1 expression in the surface of CD4＋ and CD8＋ T lymphocytes was also determined by flow cytometry.The data were statistically analyzed.Results The percentage of PD-1 expression in CD4＋ and CD8＋ T lymphocytes of chronic periodontitis group [（16.7 ± 5.5） ％,（20.8 ± 5.1） ％] and chronic gingivitis group [（14.2 ± 6.1） ％,（14.5 ± 4.3） ％] were higher than that of healthy controls [（9.5 ± 2.1） ％,（8.1 ± 1.9） ％] （P ＜ 0.05）.The percentage of PD-L1 expression in CD4＋ and CD8＋ T lymphocytes of chronic periodontitis group [（24.2 ± 7.1） ％,（15.3 ± 6.8） ％] and chronic gingivitis group [（12.4 ±6.0） ％,（11.2 ± 5.5） ％] were higher than that of healthy controls [（4.7 ± 1.2） ％,（3.2 ± 2.3） ％] （P ＜ 0.05）.The percentage of PD-1/PD-L1 expression in CD4＋ T lymphocytes and CD8＋ T lymphocytes of the chronic periodontitis group were significantly decreased after initial therapy（P ＜ 0.05）.Conclusions The expression of PD-1 and PD-L1 in peripheral CD4＋ T lymphocytes and CD8＋ T lymphocyte of chronic periodontitis patients was up-regulated and was associated with periodontal condition.The initial therapy reduced the expression of PD-1 and PD-L1.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2014年第4期216-219,共4页
Chinese Journal of Stomatology
