摘要
目的:了解广西地区丙型肝炎病毒(hepatitis C virus,HCV)基因型的分布特征,探讨HCV基因型与民族、性别、年龄、感染途径及HCV RNA载量的关系.方法:收集广西地区137例HCV RNA阳性患者的血清样本,采用逆转录巢式PCR(RT nested-PCR)法扩增HCV NS5B区段,对PCR终产物纯化测序后与Genbank中的标准株全基因序列比对,共同构建HCV NS5B区段系统进化树.结果:广西地区HCV基因型主要为1b型(58.4%),其次是6a型(12.4%),3b型(10.2%),1a型(7.3%),2a型(7.3%),3a型(4.4%).分析表明,HCV基因型与民族、性别无明显相关性(P>0.05),而与年龄、感染途径及HCV-RNA载量有一定相关性(P<0.05).结论:构建HCV NS5B区段系统进化树能得到准确的HCV基因型和亚型;广西地区至少存在7种HCV基因亚型,以1b型为主,6a型和3b型次之;较国内早期的研究报道,1b型的流行率似乎有下降趋势,而6a型的流行率似乎逐步上升;3型与6a型多见于有静脉药瘾史的年轻患者;随着感染途径的变迁,HCV基因型的分布也将发生改变.
AIM： To investigate the distribution characteris- tic of HCV genotypes in Guangxi, and to explore the correlations of ethnicity, gender, age, route of infection and HCV-RNA load with HCV gen- otypes. METHODS： The NS5B region was amplified from137 HCV-RNA positive serum samples and se- quenced. The sequences obtained were compared with the sequences deposited in Genebank to construct a HCV NS5B phylogenetic tree. RESULTS： The major genotype of HCV in Guangxi was lb （58.4%）, followed by 6a （12.4%）, 3b （10.2%）, 1a （7.3%）, 2a （7.3%）, and 3a （4.4%）. There were no significant correlations between HCV genotype and ethnicity or gender （P 〉 0.05 for both）. Significant correlations were found be- tween HCV genotype and age, route of infection and HCV-RNA load （P 〈 0.05 for all）. CONCLUSION： Accurate HCV genotypes and subgenotypes can be obtained by establishing a phylogenetic tree of the HCV NS5B region. At least seven subgenotypes were detected in Guangxi and the main genotypes are 1b, 6a and 3b. Compared with previous reports, our results indicated that the prevalence of genotype lb seems to decline, and the prevalence of 6a tends to increase gradually. Genotypes 3 and 6a were mainly found in young intravenous drug us- ers. The distribution of HCV genotypes changes with the change in the route of infection.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第9期1300-1306,共7页
World Chinese Journal of Digestology
基金
广西卫生厅基金资助项目
No.Z2011346~~
