摘要
目的:观察麦冬多糖对NOD小鼠颌下腺的保护作用。方法:给予NOD小鼠麦冬多糖灌胃,并设置羟氯喹阳性对照组,最后用ELISA法检测相关血清细胞因子,并对小鼠的颌下腺做HE染色,用RT-PCR法检测颌下腺IFN-γ与IL-10mRNA的表达水平。结果:和模型相比,麦冬多糖能降低颌下腺淋巴细胞浸润度,并可修复Th1/Th2细胞因子失衡。结论:麦冬多糖对NOD小鼠颌下腺具有保护作用。
Objective：To detect the effect of Ophiopogon japonicus on submandibular glands in NOD mice.Methods：Ophiopogon japonicus polysaccharides（OJP） was administered and hydroxychloroquine was served as positive control.Serum cytokines were detected by enzyme-linked immunosorbent assay （ELISA）.Histological changes in the submandibular glands （SMG） were examined by hematoxylin and eosin （HE） staining.The messenger ribonucleic acid （mRNA） expression of gamma-interferon （IFN-γ） and interleukin-10 （IL-10） were measured with RT-PCR method.Results：Compared to the model mice,the extent of the histological changes of the mice administrated with Ophiopogon japonicus was ameliorated,the Th1/Th2 cytokine imbalance was remedied.Conclusion：Ophiopogon japonicus could protect the submandibular glands in NOD mice.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2014年第2期198-201,共4页
Chinese Journal of Immunology
基金
国家自然科学基金项目(No.81102525/H2701)
江苏省南京市卫生局医学科技发展一般性课题(No.YKK11043)
南京中医药大学校级开放课题(No.zynk003)
江苏省普通高校研究生科研创新计划项目(No.CXZZ13_0608)
关键词
麦冬多糖
干燥综合征
颌下腺
Ophiopogon japonicus
Sj（o）gren＇s syndrome
Submandibular glands
