摘要
目的：建立仙人掌中芦丁含量的高效液相色谱（HPLC）测定方法。方法：以芦丁为对照品,采用色谱柱为ODS C18色谱柱（250mm×4.6mm,5μm）,以甲醇：0.4%磷酸（40：60）为流动相,流速为1mL/min,柱温25℃,进样量10μL,检测波长为360nm。结果：芦丁的进样量范围在0.5-5μg内（r=0.999 9）,线性关系良好;平均回收率为100.527%（RSD为1.970%）。结论：该方法简便、灵敏、可行、稳定,分离效果较好。
Objective： High performance liquid chromatograpghy（ HPLC） was used to determine the content of Rutin in Oputia dillenii haw. Method： Chromatograph conditions were as follows： ODS- SP（ 250mm × 4. 6mm,5μm）,mobile phase was methanol： 0. 4% Phosphate（ 40： 60）,the flowrate was 1mL / min,column temperature was 25℃,the injection volume was 10μL,λ = 360nm. The detection wavelength was 360 nm. Result： The good liner rang of rutin was within 0. 5 ~ 5μg,r = 0. 999 9; and the recovery rate was 100. 527%（ RSD = 1. 970%）. Conclusion： The method is simple, sensitive,viable and stable. Moreover,it has a better separative effect.
出处
《中医药学报》
CAS
2014年第1期22-24,共3页
Acta Chinese Medicine and Pharmacology
基金
新疆维吾尔自治区重点学科食品科学青年教师科研启动项目
