摘要
目的 :采用薄层扫描法测定痛立克注射液中延胡索乙素、粉防己碱和防己诺林碱的含量。方法 :采用日本岛津CS - 930 1型双波长飞点扫描仪 ,检测波长为 36 0nm。结果 :延胡索乙素点样量在 0 4～ 2 2 μg ,粉防己碱在 0 5～ 2 5 μg ,防己诺林碱在 0 3～ 1 5 μg的范围内与峰面积呈良好的线性关系 ,r分别为 0 997,0 998,0 996 ,回收率分别为 96 6 % ,96 6 % ,97 7%。结论 :本方法操作简便 ,精密度好 ,结果准确可靠。
Objective:To determine tetrahydropalmatine,tetrandrine and demethyltetrandrine in Tonglike injection by TLCS.Methods:The CS-9301 dual wavelength flying spot scanning densitometer was selected.The detection wavelength was 360 nm.Results:The calibration curves were linear in the range of 0 4～2 2 μg for tetrahydropalmatine( r =0 997),0 5～2 5 μg for tetrandrine( r =0 998)and 0 3～1 5 μg for demethyltetrandrine( r =0 996)respectively.The average recoveries( n =5)were 96 6% for tetrahydropalmatine(RSD=3 1%),96 6% for tetrandrine(RSD=3 4%)and 97 7% for demethyltetrandrine(RSD=5 0%).Conclusion:The method is rapid,accurate and reliable.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2001年第1期28-30,共3页
Chinese Journal of Pharmaceutical Analysis
