摘要
目的建立测定复方思日颗粒中绿原酸含量的方法。方法采用Waters Sun Fire TMCis（150mm×4．6mm，5μm）色谱柱，流动相为甲醇（A）-乙腈（B）-0．4％磷酸水溶液（C），梯度洗脱（0～50min，5．5％A-6％A，2．9％B，91．6％C→91．1％C），柱温30℃，流速1．0mL／min，检测波长327nm，进样量15μL.结果绿原酸对照品在4．24～25．44μg／mL浓度范围内与峰面积的线性关系良好。加标回收率为98．739％（n=9），RSD为2．622％，分离度〉1．5，对称因子不得低于0．7。结论HPLC方法简便、结果准确，可用于复方思日颗粒中绿原酸的含量测定。
Objective To establish a HPLC method to determine the content of Chorogenic acid in Si Ri Granules. Method A Waters SunFire TM Cls （150 mm×4.6 mm, 5μm） column was used. The mobile phase was methanol（A）-acetonitrile（B）--0.4%H3PO4（C）（0～50 rain,5.5% A→6% A,2.9%B,91.6% C →91.1% C）with gradient elution and detection wavelength was 327 nm. The flow rate was 1.0 mL/min, the injection volume was 15 μL and the column temperature was 30℃. Result A good linearity was obtained in the range of 4.24～25.44μg/mL. The average recovery was 98.739%（RSD 2.622%, n =9）. The Separating size was more than 1.5, the symmetrical factor was not less than 0.7. Conclusion The method was good for determining Chorogenic acid in Si Ri Granules.
出处
《新疆医科大学学报》
CAS
2014年第4期443-445,449,共4页
Journal of Xinjiang Medical University
基金
国家联合基金项目(U1203203)
