期刊文献+

自体骨髓间充质干细胞微创治疗骨不连疗效分析 被引量:2

下载PDF
导出
摘要 目的探讨经皮自体骨髓间充质干细胞(BMSCs)移植术治疗四肢骨折非感染性骨不连的疗效。方法选取四肢骨折骨不连患者16例,自体髂骨获取其BMSCs后进行培养,经皮注射于骨不连部位。术后定期x线检查观察骨折断骨痂生长情况。结果15例愈合。全部病例得到随访,术后随访10~18个月,骨痂出现时间(8.2±2.5)周,骨折愈合时间(4.5±1.6)个月;上肢骨折按照临床上肢评分系统标准,胫骨骨折按照Johner-Wruhs功能分级法评定标准,股骨骨折按照Schatzker-Lamben疗效评分系统等评估患肢恢复情况,优12例、良2例、可2例。均未发生注射创口化脓性感染、血肿、深静脉血栓形成、肌腱损伤、神经损伤以及固定材料失效与断裂等不良并发症。结论微创经皮自体BMSCs移植治疗非感染性四肢骨折不愈合有效而且安全。 Objective To explore the therapeutic effects of treating none infectional fracture nonunion with bone mesenchymal stem cells (BMSCs) from autologous bone marrow. Methods 16 patients suffering from fracture nonunion after limb fracture were enrolled in this study. BMSCs were aquired from their own iliac bone and cultured before they were percutaneously injected into the site of fracture nonunion. Regular X ray was used to observe the growth of bone callus at the fracture. Results 15 patient got healed. All the patients were followed up for 10-18 months, it took ( 8.2 ~ 2.5 ) weeks for the bone callus to appear, the fracture healed on ( 4.5 ~ 1.6 ) months.For the evaluation of fracture healing, the fracture of upper limb was according to the clinical scoring system of upper limb and the tibial fracture was according to Johner-Wruhs functional grading standard, the femoral fracture was according to Schatzker-Lambert scoring system, 12 cases were excellent, 3 cases were good, 2 cases were medium. No pyogenic infection, hematoma, DVT, tendon injurey, nerve injure or the complications related to the materials for fixing ocurred. Conclusions Treating none inflectional fracture nonunion minimally invasively with BMSCs from autologous bone marrow is effective and safe.
出处 《浙江临床医学》 2014年第4期519-520,523,共3页 Zhejiang Clinical Medical Journal
基金 温州市科技局基金项目(编号Y20130098)
关键词 骨髓间充质干细胞 微创 治疗 骨不连 Bone mesenchymal stem cells Minimally invasive surgery Treatment Fracture nonunion
  • 相关文献

参考文献9

  • 1Borrelli J Jr,Prickett WD,Ricci WM.Treatment of nonunions and ossesous defcts with bone graft and calcium sulfate.ClinOthop Relat Res,2003(411):245-254.
  • 2Teseng SS,Lee MA,Reddi AH.Nonunions and the potential of stem cells in fracture healingJ Bone surg Am,2008,90(suppl),92-98.
  • 3Koob S, Torio-Padron N, Stark B, et al. Bone formation and neovascularization mediated by mesenchymal stem cells and endothelial ceUs in critical-sized calvarial defects,Tissue Eng Part A,2011,17(3-4): 311-321.
  • 4童培建,厉驹,瞿杭波,陈峥嵘.经皮自体骨髓间充质干细胞移植治疗四肢骨折骨不连[J].中华创伤杂志,2007,23(7):499-501. 被引量:25
  • 5Marti RK,Verheyen CC,Besselaar PP.Humeral shaft nonunion evalution of uniform surgical repair in fifty-one patients,J,J Trauma,2007, 19(1): 108-115.
  • 6infarction: double-blind, randomised controlled trial. Lancet, 2006, 367(9505): 113-121.
  • 7Ma HL,Chen TH,Hung SC,Development of new method in promoting fracture healing:multiple cryopreserved bone marrow injections using a rabbit model.Arch Orthop Trauma Surg,2004,124(7):448-454.
  • 8Zhao L,Jiang S,Hantash BM,Transforming growth factor betal induces osteogenic differentiation of murine bone marrow stromal cells,Tissue Eng Part A,2010,16(2):725-733.
  • 9Herberts CA,Kwa MS,Hermsen HP.Risk factors in the development of stem cell therapy,J Transl Med,2011 (9) :29-34.

二级参考文献10

  • 1Prockop DJ,Marrow stromal cells as stem cells for nonhematoopoietic tissues.Science,1997,76:71-74.
  • 2Pittenger MF,Mackay AM,Beck SC.Multilineage potential of adult human mesenchymal stem cells.Science,1999,284:143-147.
  • 3Shi XM,Blair HC,Yang X,et al.Tandem repeat of C/EBP binding sites mediates PPAR gamma 2 gene transcription inglucocorticoid-induced adipocyte differentiation.J Cell Biochem,2000,76:518-527.
  • 4Hernigou A,Poignard F,Beanjean H,et al.Percutaneous autologous bone-marrow grafting for nonunions.J Bone Joint Surg(Am),2005,87:1430-37.
  • 5Soltan M,Smiler DG,Gailani F,A new“platinum”standard for bone grafting:autogenous stem cells.Implant Dent,2005,14:322-325.
  • 6Connolly JF,Guse R,Tiedeman J,et al.Autologous marrow injection as a substitnte for operative grafting of tibial nonunions.Clin Orthop,1991,(266):259-270.
  • 7Healey JH,Zimmerman PA,McDonnell JM,et al.Percutaneous bone marrow grafting of delayed union and nonunion in cancer patients.Clin Orthop,1990,(256):280-285.
  • 8Johnson KD.Management of malunion and nonunion of the tibia.Orthop Clin North Am,1978,18:157-171.
  • 9Knop I,Sitte F,Canto M,et al.Successful posterior interlaminar fusion at the thoracic spine by sole use of β-tricalcium phosphate.Arch Orthop Trauma Surg,2006,126:204-210.
  • 10梁雨田,张伯勋,卢世璧,陶笙,肖嵩华.经皮自体骨髓移植治疗骨折不愈合[J].中华骨科杂志,1999,19(12):709-711. 被引量:97

共引文献24

同被引文献14

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部