摘要
三疣梭子蟹是中国重要的水产养殖动物。目前，其养殖业的蟹苗主要还是依靠野生资源，但种质（生长速度、肌肉品质和抗病性）退化现象严重，因此迫切需要了解个体、种群和物种遗传多样性。报道了一种检测三疣梭子蟹多样性芯片技术（diversityarraystechnology，DART）。DArT技术采用芯片可同时检测数百个基因座，即使没有基因组DNA序列信息也可以分析遗传多态性。采用了风tL／TaqI复杂性降低方法，多态性效率为12．5％。利用多样性芯片杂交获得313个DArT标记。这些DArT标记用于点制多态性富集芯片，DArT标记具有较高的多态性信息含量（polymorphisminformationcontent，PIC）。最后根据0／1矩阵获得UPGMA系统进化树，结果表明样品间的遗传多样性与文献报道一致。芯片质量检测中包括赋值重复性为99．1％，同一样品不同个体间的差异不显著，其差异介于0．7％～1．6％。研究表明，DArT技术具有高通量和低成本的显著特点，该技术可为三疣梭子蟹资源保护和良种选育提供支持。
The swimming crab, Portunus trituberculatus, is an important farmed species in China. Commercial crab farming now largely depends on wild seed stock. The commercial characteristics （growth rate, flesh quality and disease resistance） of the cultured stocks have declined seriously after many years of culturing. It is crucial and urgent to understanding the distribution of genetic diversity within and among individuals, populations and species. Here the development of Diversity Arrays Technology （DART） was reported for swim- ming crab. DArT using microarrays can be used to detect DNA polymorphism at several hundreds genomic loci in a single assay without relying on DNA sequence information. The Pst I/ Taq I complexity reduction methods were used. And the frequency of polymorphic clones was 12. 5%. 313 polymorphic clones were detected on the discovery arrays. The polymorphism-enriched Pst I/Taq I arrays were also produced by using 313 polymorphic clones. DArT markers displayed fairly high polymorphism information content （PIC） values. A UPGMA dendrogram constructed from 313 Pst I/Taq I markers. The dendrogram revealed the genetic relationships among the sam- ples were consistent with the reported references. The scoring reproducibility was 99. 1%. Very few differences observed among pairs of individuals in the same DNA samples were in the range of 0. 7% - 1.6%.
出处
《生物学杂志》
CAS
CSCD
2014年第2期18-21,63,共5页
Journal of Biology
基金
浙江省海洋生物技术产业科技创新团队(2012R10029-06)
浙江省大学生新苗人才计划项目(2012R405042)
宁波市农业与社会发展攻关项目(2010C10019)
关键词
三疣梭子蟹
DART
地理种群
遗传多样性
P. trituberculatus
DART
geographical populations
genetic diversity
