摘要
目的:声触诊量化技术(virtual touch tissues quantifleation,VTQ)在小鼠肝纤维化诊断中的实验研究.方法:40只小鼠随机分成对照组、CCl4 1 wk组、CCl4 4 wk组和CCl4 8 wk组.于造模时间点测定VTQ测量值(Vs值);检测门冬氨酸氨基转移酶(aspartate aminotransferase,AST)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、Ⅳ型胶原(Ⅳcollagen,Ⅳ-C)、透明质酸(hyaluronic acid,HA)和层黏连蛋白(laminin,LN)的变化;制作肝组织石蜡切片,观察病理学变化.结果:HE染色显示对照组肝小叶结构正常;1wk时可见肝细胞少量坏死,炎症细胞少量浸润;4 wk时肝细胞气球样变明显,可见不同程度碎屑坏死;8 wk时正常肝小叶结构消失,大量炎症细胞浸润.Masson染色显示对照组仅在中央静脉壁上少量绿色胶原纤维;1 wk时中央静脉及汇管区可见绿色胶原纤维;4 wk时胶原纤维间隔较明显;8 wk时大量增生的胶原纤维包绕、分割成大小不等的假小叶.血清学AST、AST、Ⅳ-C、LN及HA检测比较:1 wk组与对照组无差别(P>0.05),4 wk组、8wk组与对照组差别具有显著性意义(P<0.01,P<0.001).VTQ测量值(VTQ value,Vs)显示1wk组与对照组无差别(P>0.05);4、8 wk组与对照组差别具有显著性意义(P<0.001).结论:与组织病理学及血清学检测一样,声触诊量化技术也可作为一种新手段在诊断肝纤维化动态变化中提供一定的参考价值.
AIM： To assess the application of virtual touchtissue quantification（VTQ） in the diagnosis of liver fibrosis in mice. METHODS： Forty male mice were randomly divided into control, CCl4-1 wk, CCl4-4 wk and CCl4-8 wk groups. At different time points, VTQ value was calculated, and aspartate aminotransferase（AST）, alanine aminotransferase（ALT）, collagen Ⅳ（Ⅳ-C）, hyaluronic acid（HA） and laminin（LN） were measured. Liver tissues were taken for pathological analysis. RESULTS： HE staining indicated that the 1-wk group showed a small amount of necrosis and inflammatory cell infiltration; the 4-wk group showed significant hepatocyte ballooning degeneration and some necrosis; the 8-wk group showed a large amount of inflammatory cell infiltration and abnormal lobular structure; and the control group had normal lobular architecture. Compared with the control group with a few collagen fibers in the vessel wall, the number of collagen fibers increased significantly in the 1-, 4- and 8-wk groups. The fibrous septa was obvious in the 4-wk group and pseudolobule formation with proliferated collagen fibers was present in the 8-wk group. Compared with the control group, AST, ALT, Ⅳ-C, LN and HA showed no significant differences in the 1-wk group（P〈0.05）, but were significantly different in the 4- and 8-wk groups（P〈0.01, P〈0.001）. VTQ value showed no significant difference between the control group and 1-wk group（P〈0.05）, but differed significantly between the control group and 4- and 8-wk groups（P〈0.01, P〈0.001）. CONCLUSION： VTQ can be used as a new method for the diagnosis of liver fibrosis.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第7期963-968,共6页
World Chinese Journal of Digestology
基金
国家自然科学基金资助项目
No.81272222
广东省科技计划基金资助项目
No.2011B031300026~~
