摘要
目的:观察洛哌丁胺构建的大鼠便秘模型中水通道蛋白家族中AQP3和AQP8蛋白及其mRNA定量表达,探讨洛哌丁胺构建大鼠便秘模型对AQP3和AQP8的影响.方法:SD♂大鼠30只,随机分为5组,造模开始时处死1组大鼠,检测结肠传输功能及结肠组织的AQP3,8表达水平,作为基准参考线.剩余4组予以洛哌丁胺1.5 mg/(kg·d)灌胃,诱导造模.分别于造模的第3、7天和造模成功后第3、7天各处死一组模型大鼠,检测结肠传输及AQP3,AQP8蛋白及其mRNA定量表达.结果:大鼠粪便含水量在诱导便秘模型的过程中明显减少,停药后7 d,粪便内的水分并不能自发恢复至正常(2.29 g±1.17 g vs 0.80 g±0.27 g,P=0.01<0.05).试验过程中,大鼠结肠传输功能未发生改变(67.72%vs 58.64%,P=0.971>0.05).AQP8远端表达停药7 d上升,与造模7 d时相比,差异有统计学意义(0.43±0.31vs 2.66±0.87,P=0.008).AQP8近端表达给药后迅速上升至较高水平,与正常组对照具有统计学意义(15.96±2.13,P=0.00),停药7 d后其表达再次上升,与给药7 d相比,差异有统计学意义(6.37±0.23 vs 0.79±0.13,P=0.015).停药后,AQP3在结肠近端持续表达下降,与正常对照组相比,差异有统计学意义(0.20±0.06,P=0.04).结论:洛哌丁胺能够提高模型大鼠结肠AQP8表达,促进肠道跨膜水运输,减少粪便含水量而导致便秘.
AIM： To observe the differential expression of AQP3 and 8 in loperamide induced constipation in rats, and to explore the role of these two proteins in constipation.METHODS： Thirty male SD rats were randomly divided into five groups. One group was killed at the beginning to measure intestinal transit function and the expression of AQP3 and 8 in human colonic epithelial cells as basic references. Loperamide [1.5 mg/（kg？d）] was given by intragastric administration in the remaining four groups of rats to induce constipation. Two groups of rats were killed on days 3 and 7 after initial loperamide administration, respectively, while the other two groups were killed 3 and 7 d after last loperamide administration, respectively. Intestinal transmission function was determined, and the expression of AQP3 and 8 was detected. RESULTS： Intestinal transit function kept stable（67.72% vs 58.64%, P〈0.05） throughout the experimental process, while fecal water content decreased. The fecal water content could not return to normal 7 d after stopping loperamide（2.29 g ± 1.17 g vs 0.80 g ± 0.27 g, P = 0.01）. AQP8 expression in the proximal colon had an upward trend 3 to 7 d after the initial loperamide administration, greatly higher than that in the normal control group（P = 0.00）. Seven days after stopping loperamide, AQP8 had a rising trend in both the proximal and distal colons（0.43 ± 0.31 vs 2.66 ± 0.87, P = 0.008）, while AQP3 expression declined（6.37 ± 0.23 vs 0.79 ± 0.13, P = 0.015）. CONCLUSION： Loperamide enhances the expression of AQP8 in the intestine of rats, which facilitates water movement across the membrane. Deficient water absorption may play a role in the pathogenesis of loperamide induced constipation.
出处
《世界华人消化杂志》
CAS
北大核心
2014年第7期969-974,共6页
World Chinese Journal of Digestology
基金
江苏省六大人才高峰重点课题基金资助项目
No.2011-WS40
国家自然科学基金资助项目
No.81373645
江苏省中医药管理局课题基金资助项目
No.LZ11042~~
