摘要
以浙江省台州紫山药带腋芽的茎段为外植体进行紫山药种苗的快速繁殖。结果表明：70％乙醇浸泡30 s及84消毒液消毒15～20 min配合使用灭菌效果最好；腋芽诱导最适培养基为MS＋0.5 mg·L-16-BA＋0.1 mg·L-1 NAA，培养25 d后诱导的芽数最多，平均芽数为1.58，高度最高，为2.3 cm；生根培养最适培养基为1/2 MS＋0.1 mg·L-16-BA＋2.0 mg·L-1 NAA＋0.02％活性炭，平均生根天数为6 d，生根率达100％，气生根率低，根系长而粗壮；生根培养基中培养30 d后，将试管苗按节切段繁殖，繁殖系数可达3.0。
With the stem segments as explants , the tissue culture and rapid propagation of purple Dioscorea alata from Taizhou, Zhejiang Province were investigated.The results showed that the sterilization efficacy of 70%alcohol for 30 s and 84 disinfector for 15-20 min was the best.The optimal axillary buds inducing medium was MS ＋0.5 mg·L-1 6-BA＋0.1 mg·L-1 NAA, in which the buds number was the largest and the height was maximum .After breeding for 25 d,the average buds number was 1.58 and the height was 2.3 cm.The optimal rooting medium was 1/2MS＋0.1 mg·L-1 6-BA＋2.0 mg·L-1 NAA＋0.02%activated carbon, in which the average rooting days was 6 d, rooting rate was 100%, aerial roots rate was low and roots were thick and strong .After 30 d culture in rooting medium, the lateral branch cuttings with the axillary buds were propagated in the rooting medium , and the multiplication coefficient reached 3.0.
出处
《浙江农业学报》
CSCD
北大核心
2014年第2期344-347,共4页
Acta Agriculturae Zhejiangensis
基金
江苏省农业科技自主创新资金[CX(13)2009]
关键词
紫山药
组织培养
腋芽诱导
purple Dioscorea alata
tissue culture
axillary buds induction
