摘要
目的观察芍药甘草汤对哮喘大鼠信号传导和转录激活因子6(STAT 6)与白介素-13(IL-13)水平的干预作用,探讨其防治支气管哮喘的作用机制。方法 60只SD雄性大鼠随机分为正常对照组(NULL组)、模型组(OVA组)、地塞米松组(DXM组)及芍药甘草汤高(SG10组)、中(SG5组)、低(SG2.5组)剂量组,每组10只。除正常对照组外均行支气管哮喘造模,相应药物灌胃。检测各组大鼠支气管肺泡灌洗液中白细胞和嗜酸性粒细胞计数,免疫组化法测肺组织中STAT 6蛋白表达,ELISA法检测血浆中IL-13含量。结果与NULL组比较,OVA组哮喘大鼠肺泡灌洗液(BALF)中白细胞总数、嗜酸性粒细胞计数及气道上皮STAT 6表达和血浆IL-13水平均明显升高(P<0.01)。DXM组及各剂量芍药甘草汤组BALF中白细胞总数和嗜酸性粒细胞计数、气道上皮STAT 6表达和血浆IL-13水平较OVA组降低(P<0.01),但仍高于NULL组(P<0.01)。结论芍药甘草汤防治大鼠支气管哮喘的作用机制可能与抑制哮喘大鼠肺组织STAT 6表达,降低血浆IL-13水平,降低哮喘大鼠BALF中白细胞总数及嗜酸性粒细胞计数有关。
Objective To observe the effect of Shaoyaogancaotang（SG） on signal transducer and activator of tran-scription 6（STAT6） and interleukin-13 in asthmatic rat, and to explore the possible mechanism of SG to asthma. Methods Sixty SD rats were randomly divided into 6 groups： normal group（group NULL）, asthmatic group（group OVA）, dexamethasone group（group DXM）, group SG10, group SG5, and group SG2.5. Before nebulization, group NULL and OVA was given saline of 10ml/kg intragastrically, group DXM was given DXM of 1 mg/kg, and groups SG were given SG of 10, 5, and 2.5 g/kg, respectively. The numbers of total leukocytes and eosinophils in bron-choalveolar lavage fluid（BALF） were measured by light microscope. The protein expression of STAT6 in airway ep-ithelium was shown by immunohistochemistry. The serum IL-13 were detected by ELISA. Results After sensitized and challenged, the numbers of total leukocytes and eosinophils in BALF of OVA-treated rats significantly in creased compared with group NULL（P〈0.01）. After treatment with different dosages of SG and dexamethasone, the numbers of total leukocytes and eosinophils in BALF, the STAT6 protein expression, and serum IL-13 were signifi-cantly lower than those in group OVA（P〈0.01）, but higher than those in group NULL（P〈0.01）. Conclusion Shaoyao-gancaotang can significantly inhibit the protein expression of STAT6 in pulmonary tissue, decrease the IL-SG can inhibit the protein expression of STAT6 in pulmonary tissue, and decrease the serum IL-13 and the numbers of to-tal leukocytes and eosinophils in BALF. SG might be of potential value in protection and treatment of asthma.
出处
《浙江中西医结合杂志》
2014年第4期287-289,共3页
Zhejiang Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省中医药优秀青年人才基金计划(No.2012ZQ013)
