摘要
目的探讨六味五灵片对脂多糖(LPS)联合D-氨基半乳糖(GalN)所致肝损伤的防护作用机制。方法将84只BALB/c雄性小鼠随机分为3组,每组28只。正常组、对照组连续7日每日给予生理盐水0.2 mL腹腔注射,观察组每日给予六味五灵片(16 g/kg体重)生理盐水2 mL灌胃,末次灌胃4 h后对照组和观察组给予LPS(5μg/kg)和GalN(400mg/kg)腹腔注射。观察造模后9、12、15、18 h,3组小鼠血浆ALT、AST及NO-2含量以及肝组织MDA含量的变化情况。结果观察组各时间点血浆ALT、AST及NO-2的含量与正常组比较差异无统计学意义(P>0.05),而与模型组比较差异均有统计学意义(P<0.05)。观察组各时间点肝组织MDA含量与正常组比较均无统计学意义(P>0.05),而与模型组比较差异均有统计学意义(P<0.05)。结论六味五灵片防护肝损伤的作用机制可能是通过抑制机体NO的生物合成从而降低NO含量、阻断脂质过氧化反应发生,从而抑制MDA过量产生实现的。
Objective To explore the protective effect mechanism of Liuwei Wuling Tablet on hepatic injury induced by lipopolysaccharide （LPS） and GAIN. Methods Eighty-four male BALB/c mice were randomly divided into three groups, 28 cases in each one. The intraperitoneal injection of normal saline （0. 2 mL） was used in the normal group and control group, once a day for continuous 7 days; the intraperitoneal injection of LPS （5 μg,/kg） and GaIN （400 mg,/kg） was used in the control group. Liuwei Wuling Tablet （ 16 mg/kg） was given in NS （2 mL） by intragastric administration in the observation group, once a day for continuous 7 days. The content of ALT, AST and NO2- as well as malondialdehyde （MDA） in the liver tissue were observed 9 h, 12 h, 15 h and 18 h after model establishment in three groups. Results The content of ALT, AST and NO2- in the observation group at each time point was not statistically different from that in the normal group （ P 〉 0. 05 ）, while compared with the model group, the difference was significant （P 〈 0. 05 ）. The MDA in the 1 iver tissuein the observation group at each time point was not statistically different from that in the normal group （ P 〉 0. 05 ）, while compared with the model group, the difference was significant （ P 〈 0. 05 ）. Conclusion It is likely that Liuwei Wuling Tablet could take a protective on hepatic injury by inhibiting the biosynthesis of NO to lower NO content and preventing the reaction of lipid peroxidation to restrain the overproduce of MDA.
出处
《北京中医药》
2014年第3期226-228,共3页
Beijing Journal of Traditional Chinese Medicine
关键词
六味五灵片
肝损伤
作用机制
一氧化氮
丙二醛
Liuwei Wuling Tablet
hepatic injury
action mechanism
nitric oxide
malondialdehyde
