摘要
目的 :探讨细胞因子γ干扰素 (IFN-γ)、肿瘤坏死因子 (TNF)和白介素 - 1(IL - 1)对人单核 /巨噬细胞表达 CD40和CD40 L的影响。 方法 :应用 RT- PCR方法半定量测定 CD40和 CD40 L m RNA含量 ,流式细胞技术检测细胞表面 CD40和CD40 L表达水平。 结果 :IFN-γ,TNF和 IL - 1既增加 CD40和 CD40 L m RNA表达 ,又能诱导单核 /巨噬细胞表面 CD40和CD40 L高表达 ,而抗氧化剂 Vit E能下调细胞因子引起的 CD40和 CD40 L表达 ,但作用不完全。结论 :细胞因子 (IFN-γ,TNF和 IL - 1)能刺激单核 /巨噬细胞高表达 CD40和 CD40 L ,这种作用可被 Vit
Objective: To investigate the effect of cytokines (IFN γ,TNF and IL 1) on the expression of CD40 and CD40 ligand (CD40L) in monocytes/macrophages. Methods: The mRNA expression of CD40 and CD40L was measured by RT PCR and the CD40,CD40L expression on the monocytes/macrophages were detected by flow cytometric analysis. Results: IFN γ,TNF and IL 1 could not only significantly up regulate the mRNA levels of CD40 and CD40L in cultured monocytes/macrophages, but also increase the expression of CD40 and CD40L. Antioxidant VitE could reduce the expression of CD40 and CD40L induced by IFN γ,TNF and IL 1. Conclusion: IFN γ,TNF and IL 1 can stimulate high expression of CD40 and CD40L . Antioxidant VitE can partially inhibit the expression of CD40 and CD40L induced by cytokines in cultured monocytes/macrophages. [
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2001年第2期127-129,共3页
Academic Journal of Second Military Medical University
基金
国家重点基础研究 (973 )项目! (G2 0 0 0 0 5 690 3 )
