摘要
目的 探讨严重烧伤早期脑水肿时脑血流的变化特点。方法 将 2 6只家犬随机分为对照组 ,烧伤后 6、12、18和 2 4h组。采用内源性碱性磷酸酶组化染色 (EAHCS)及图像分析方法观察脑组织毛细血管形态和分布 ,并用体视学分析方法测定脑毛细血管的体积、面积和长度密度分数。结果 烧伤后 6h和 12h毛细血管分布密度逐渐增加 ,血管形态无明显改变 ;伤后 18h和 2 4h毛细血管分布密度趋于减少 ,血管形态出现枯藤状改变。体视学分析发现 ,伤后 6h和 12h毛细血管密度分数趋于增加 ,而伤后 18h和 2 4h毛细血管密度分数明显减少 ,于伤后 2 4h达最低值 (P <0 .0 5 )。结论 提示脑血流量的变化在严重烧伤脑水肿的病理过程中起着重要作用。
Objective To explore the characteristics of cerebral blood flow during cerebral edema at the early stage of severe burn in dogs. Methods A total of 26 dogs was randomized into control, 6, 12, 18 and 24 h postburn groups. The shapes and distribution of the cerebral capillaries were observed with the endogenous alkaline phosphatase histochemical staining (EAHCS)and image analysis. The volume, area and length fractions of cerebral capillaries were measured and analyzed with stereographic method. Results The distributional density of capillaries was increased gradually in 6 and 12 h postburn groups, but little change of capillary shape was found at these time points. However, the distributional density of capillaries became sparser at 18 h and 24 h after burn, and withered vine like capillaries appeared. With stereolographic analysis, the capillary volume, surface and length fractions were increased in 6 and 12 h group, but decreased at the 18 and 24 h, and reached the lowest point at the 24 hour postburn ( P <0.05). Conclusion Results suggest that the changes of cerebral blood flow may play an important role in the process of brain edema at the early stage of severe burn.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第2期179-181,共3页
Journal of Third Military Medical University
基金
重庆市科技计划(应用基础研究 )资助项目
