摘要
目的建立同时测定栀子金花丸中绿原酸、栀子苷、黄芩苷、小檗碱、汉黄芩苷、黄芩素、芦荟大黄素、汉黄芩素、大黄素、大黄酚、大黄素甲醚11种成分的UPLC法。方法采用UPLC法，AcquityUPLCBEHC18色谱柱（100mm×2．1mm，1．7μm）；乙腈（A）-0．1％磷酸水溶液（B）为流动相，梯度洗脱；体积流量为0．4mL／min；柱温35℃；检测波长：绿原酸为324nm，栀子苷为238nm，小檗碱为345nm，黄芩苷、汉黄芩苷、黄芩素、汉黄芩素为276nm，芦荟大黄素、大黄素、大黄酚、大黄素甲醚为254nm。结果被测定的11种成分色谱峰均有良好的分离度，方法精密度、重复性的RSD均小于2％；在室温条件下24h内稳定；各成分均有较宽的线性范围和良好的线性关系（r≥0．9995）；平均加样回收率98．68％～100．47％，RSD均〈1．5％。结论本方法操作简便，测定结果准确可靠，可用于栀子金花丸的质量控制。
Objective To establish a UPLC method for the simultaneous determination of 11 components, such as chlorogenic acid, geniposide, baicalin, berberine, wogonoside, baicalein, aloe-emodin, wogonin, emodin, chrysophanol, and physcion in Zhizi Jinhua Pill （ZJP）. Methods The chromatographic separation was achieved on an Acquity UPLC BEH C18 column （100 mm × 2.1 mm, 1.7 μm） with acetonitrile （A）-0.1% phosphate acid solution （B） as mobile phase at the flow rate of 0.4 mL/min for gradient elution; The column temperature was 35 ℃. The determination wavelengths were 324 nm for chlorogenic acid, 238 nm for geniposide, 345 nm for berberine, 276 nm for baicalin, wogonoside, baicalein, and wogonin, and 254 nm for aloe-emodin, emodin, chrysophanol, and physcion. Results The 11 compounds were well separated. The RSD values of precision and reproducibility were all less than 2%. The stability was good in 24 h. The linear relationship between the concentration and peak areas of the 11 compounds was all linear （r 〉 0.9995）. The average recoveries were between 98.68%-- 100.47% and the RSD values were all less than 1.5%. Conclusion The method is simple, reliable, and accurate, and could be used for the quality control of ZJP.
出处
《中草药》
CAS
CSCD
北大核心
2014年第7期955-959,共5页
Chinese Traditional and Herbal Drugs
基金
重庆市科技攻关项目(CSTC2012ggB10001)
