摘要
本文旨在观察18β-甘草次酸(18β-glycyrrhetintic acid,18β-GA)对KCl和苯肾上腺素(phenylephrine,PE)诱导的离体大鼠肾脏叶间动脉(renal interlobar artery,RIA)收缩的影响。急性分离大鼠RIA,应用压力肌动图技术,观察18β-GA预处理后大鼠RIA对内皮非依赖血管收缩剂KCl和PE的反应情况。用微动脉段标本的全细胞膜片钳技术,观察100μmol/L 18β-GA对血管段上平滑肌细胞的膜电容(Cinput)、膜电导(Ginput)和膜电阻(Rinput)的影响。结果显示,KCl(30～100 mmol/L)和PE(0.1～30μmol/L)均可以引起大鼠RIA浓度依赖的收缩;100μmol/L的18β-GA预处理后,KCl和PE对大鼠RIA的缩血管作用明显降低(P<0.01)。给予100μmol/L 18β-GA后,RIA血管段上平滑肌细胞的Rinput、Ginput和Cinput与单个平滑肌细胞数值十分接近。以上结果提示,18β-GA可抑制KCl和PE对大鼠RIA的收缩作用,其机制可能涉及18β-GA对缝隙连接的抑制。
The aim of the present study is to investigate the effect of 1813-glycyrrhetinic acid （18β-GA） on KC1- and PE-induced con- striction of rat renal interlobar artery （RIA）. Pressure myograph system was used to observe the constriction induced by KC1 and PE （endothelial independent vasoconstrictor） in acutely separated RIA of Wistar rats with or without 18β-GA pretreatment. Whole-cell patch clamp recordings were used to observe the effect of 1813-GA on membrane input capacitance （Ci,put）, membrane input conduct- ance （Ginput） or membrane input resistance （Rinput） of smooth muscle cells embedded in arteriole segment. The results showed that both KC1 （30-100 mmol/L） and PE （0.1-30 μmol/L） induced contraction of RIA in a concentration-dependent way. After pretreatment with 1813-GA （100 μmol/L）, KC1- or PE-induced constriction of RIA was significantly decreased. After application of 1813-GA （100 gmol/L）, the Cinput, Ginput and Rinput of the in situ smooth muscle cells were very close to those of dispersed single smooth muscle cells. These results suggest 18β-GA inhibits the contraction induced by KC1 and PE, and the underlying mechanism may involve the inhibitory effect of 18β-GA on gap junction.
出处
《生理学报》
CAS
CSCD
北大核心
2014年第2期195-202,共8页
Acta Physiologica Sinica
基金
supported by National Basic Research Development Program of China(No.2012CB26611)
the National Natural Science Foundation of China(No.31260247)
the Science and Technology Research Project of Xinjiang Production and Construction Corps
China(No.2012BA021
2010GG34)
