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p53和自噬相关基因Beclinl在唾液腺多形性腺瘤和癌在多形性腺瘤中的表达及意义 被引量:5

Expression and clinical significance of p^(53) and autophagy-related gene Beclinl in salivary pleomorphic adenoma and carcinoma in pleomorphic adenoma
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摘要 目的:探讨p53、自噬相关基因Beclin1的表达及与唾液腺多形性腺瘤之间的关系。方法:应用免疫组化方法检测108例唾液腺多形性腺瘤、20例癌在多形性腺瘤中的p53及自噬相关基因Beclin1的表达,并结合临床病理因素进行分析。采用SPSS20.0软件包对数据进行统计分析。结果:p53在多形性腺瘤中的阳性率(9%)显著低于癌在多形性腺瘤中(14%)(P<0.001);Beclin1在多形性腺瘤中的阳性率(91%)则显著高于癌在多形性腺瘤中(11%)(P<0.001),但2种基因的表达与患者的性别、年龄、生长时间、肿瘤大小、位置和复发等(P>0.05)无显著相关性。p53与Beclin1在多形性腺瘤中的表达呈负相关(r=-0.330,P<0.05)。结论:p53与Beclin1蛋白表达水平与多形性腺瘤发生、发展关系密切。肿瘤发生过程中,细胞自噬作用降低和抗凋亡作用增强同时并存,提高自噬能力将成为肿瘤治疗的又一途径。 PURPOSE: To investigate the expression of p53 and Beclinl in salivary pleomorphic adenoma (PA) and its clinical significance. METHODS: The expression of p53 and Beclinl were assessed by immunohistochemistry in 108 cases of PA and 20 cases of carcinoma in pleomorphic adenoma (CIPA). The results were used to analyze the relationship between gene expressions and the development of PA as well as the clinical pathological features. Statistic analysis was conducted with SPSS 20.0 software package. RESULTS: The positive expressions of p53 in PA samples (9%) were significantly lower than that in CIPA (14%) (P〈0.001). The positive expressions of autophagy-related gene Beclinl in PA samples (91%) were significantly higher than that CIPA (11%) (P〈0.001). The expression levels of these genes were not associated with gender, age, clinical course, tumor size, and location of PA (P〉0.05). There was a negative correlation between p53 and Beclinl expression in PA (r---0.330,P〈0.05). CONCLUSIONS: The expression levels of Beclinl and p53 protein are closely related to the development of PA. Reduced autophagy and enhanced anti apoptosis coexist in the process of tumor formation. Thus, raising the autophagy ability may become another alternative choice for cancer therapy,
出处 《上海口腔医学》 CAS CSCD 北大核心 2014年第2期214-218,共5页 Shanghai Journal of Stomatology
关键词 自噬 P53 BECLIN1 多形性腺瘤 Autophagy p53 Beclinl Pleomorphic adenoma
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参考文献12

  • 1Henriksson G, Westrin KM, Carlsoo B, et al. Recurrent primary pleomorphic adenomas of salivary gland origin: intrasurgical rupture, histopathologic features, and pseudopodia [J]. Cancer, 1998, 82(4): 617-620.
  • 2Pinkston JA, Cole P. Incidence rates of salivary gland tumors: results from a population-based study [J]. Otolaryngol Head Neck Surg, 1999, 120(6): 834-840.
  • 3Stennert E, Wittekindt C, Klussmann JP, et al. Recurrent pleo- morphic adenoma of the parotid gland: a prospective histopatho-logical and immunohistochemical study [J]. Laryngoscope, 2004, 114 (1): 158-163.
  • 4刘宁,娄金丽,李宁.p53基因治疗肿瘤的研究进展[J].北京医学,2010,32(9):751-753. 被引量:9
  • 5Karja VJ, Syrjanen KJ, Kurvinen AK, et al. Expression and mutations of p53 in salivary gland tumours[J]. J Oral Pathol Med, 1997, 26(5): 217-223.
  • 6王树彦,周易明,陈宗祐.自噬与肿瘤的发生和治疗研究进展[J].复旦学报(医学版),2008,35(6):928-931. 被引量:25
  • 7梁巧青,冯震博.自噬基因Beclin1的研究现状[J].当代医学,2010,16(9):21-22. 被引量:11
  • 8Nomura H, Uzawa K, Yamano Y, et al. Overexpression and altered subcellular localization of autophagy-related 16-like 1 in human oral squamous -cell carcinoma:correlation with lymphovascular invasion and lymph-node metastasis [J]. Hum Pathol, 2009, 40(1): 83-91.
  • 9Zong WX, Moll U. p53 in autophagy control[J]. Cell Cycle, 2008, 7(19): 2947.
  • 10Maiuri MC, Galluzzi L, Morselli E, et al. Autophagy regulation by p53 [J]. Curr Opin Cell Biol, 2010, 22(2): 181-185.

二级参考文献44

  • 1黄平,刘岩峰,托娅,张平,丁存晶,方杰,王振原.大鼠弥漫性脑损伤后S100β表达与损伤时间关系[J].法医学杂志,2006,22(1):4-6. 被引量:10
  • 2Kuma A,Hatano M, Matsui M, et al. The role of autophagy during the early neonatal starvation period[J]. Nature, 2004, 432:1032- 1036.
  • 3Hara T,Nakamura K, Matsui M, et al. Suppression of basal autophagy in neural cells causes neurodegenerative disease in mice[J]. Nature,2006,441:885 - 889.
  • 4Yoshimori T. Autophagy: a regulated bulk degradation process inside cells [J ]. Biochemical & Biophysical Res Communication ,2004,313:453 - 458.
  • 5Pua HH,Dzhagalov I, Chuck M,et al. A critical role for the autophagy gene Atg5 in T cell survival and proliferation[J]. J Exp Med ,2007,204:25 - 31.
  • 6Lum J J, Bauer DE, Kong M,et al. Growth factor regulation of autophagy and cell survival in the absence of apoptosis[J]. Cell,2005,120 : 237 - 248.
  • 7Klionsky DJ, Cregg JM, Dunn WA, et al. A unified nomenclature for yeast autophagy-related genes [ J ]. Developmental Cell ,2003,5(4) :539 - 545.
  • 8Mizushima N, Yamamoto A, Hatano M, et al. Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells[J]. J Cell Biology ,2001,152(4) :657 - 667.
  • 9Kirisako T,Baba M, Ishihara N, et al. Formation process of autophagosome is traced with Apg8/Aut7p in beast[J]. J Cell Biology, 1999,147(2) :435 - 446.
  • 10Kanzawa T,Zhang L, Xiao L, et al. Arsennic trioxide induces autophagic cell death in malignant glioma cells by upregulation of mitochondrial cell death protein BNIP3 [J ]. Oncogene, 2005,24(6) :980- 991.

共引文献42

同被引文献98

  • 1吴国英,朱玲,冯振卿,彭韬,祁兵.人口腔黏膜癌变不同阶段端粒酶逆转录酶及p53蛋白的表达[J].上海口腔医学,2005,14(1):51-54. 被引量:4
  • 2段振玲,彭芝兰,王赞宏,闫乃红.自噬基因Beclin1与上皮性卵巢癌发生、发展的相关性研究[J].癌症,2007,26(3):258-263. 被引量:26
  • 3向国林,朱声荣,陈卫民,陶学金,汤国雄,蔡传宝.COX-2、cyclinD_1在多形性腺瘤和癌在多形性腺瘤中的表达及意义[J].临床口腔医学杂志,2007,23(3):131-134. 被引量:7
  • 4张志愿.口腔颌面外科学[M].北京:人民卫生出版社,2012.
  • 5Tsai WC, Lin CK, Lee HS, et al. Discordance between EGFR expression and clinicopathologic parameters of colorectal adenocarcinoma in Taiwan[J].Chin J Physiol,2012,55(5):352-360.
  • 6Ghavam-Nasiri MR, Rezaei E, Ghafarzadegan K, et al. Expression of p53 in colorectal carcinoma: correlation with clinicopathologic features[J]. Arch Iran Med,2007,10(1): 38-42.
  • 7Sandberg AA,Meloni-Ehrig AM. Cytogenefics and genetics of human cancer:methods and accomplishments[ J ]. Cancer Genet Cytogenet, 2010:,203 (2) : 102 -126.
  • 8Kishi M, Nakamura M, Nishimine M, et al. Loss of hetemzygosity on chromosome 6q correlates with decreased thrombospondin-2 expression in human salivary gland carcinomas [ J ]. Cancer Sci, 2003,94(6) :530-535.
  • 9H0njoN, Gundtz 1, Fukhshima K,et aL Comprehensive loss ofheterozygosity analysis and identification of a novel hotspot at 3p21 in salivary gland neoplasms [ J ]. Otolaryngol Head Neck Surg, 2007,137( 1 ) :119-125.
  • 10Giefiug M, Wierzbicka M, Rydzaniez M, et al. Chromosomal gains and losses indicate oncogene and tumor suppressor gene candidates in salivary gland tumors[ J]. Neoplasma,2008,55 ( 1 ) :55-60.

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