摘要
通过基因克隆技术从绿脓杆菌基因组中扩增出其外毒素基因PE40.利用基因重组技术,将PE40与已克隆的抗人乳腺癌单链抗体基因M4G3/ScFv[1]相互连接,构建出完整的抗人乳腺癌重组免疫毒素M4G3/ScFv-PE40融合基因,导入表达载体PET21a进行初步表达,得到重组蛋白M4G3/ScFv-PE40,体外细胞亲和性实验证实其可特异性识别并结合T47D人乳腺癌细胞,为进一步的融合蛋白高效表达及细胞毒实验打下基础,可望为乳腺癌的临床治疗提供一种新的靶向药物.
The PE40fragment was amplified by PCR from genome of pseudomonas exotoxin A and connected with cloned single-chain antibody against human breast cancer gene which named M4G3/ScFv.The fusion gene was cloned into an expression vector PET21.After induced by IPTG,the product was detected by SDS-PAGE analysis and immunofluoresence.This experiment was expected to provide a new targeted drug for the clinical treatment of breast cancer.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第6期64-69,共6页
Acta Scientiarum Naturalium Universitatis Nankaiensis
基金
天津市自然科学基金(013609111)
国家自然科学基金(81101567)
