期刊文献+

HP-PRRSV HuN4株及其致弱过程中的不同代次病毒对仔猪外周免疫器官及肺脏的损伤 被引量:1

Study on the lesions of peripheral immune organs and lung in the piglets infected with HP- PRRSV HuN4 strain and its viral strains from different generations in the course of its attenuation
原文传递
导出
摘要 为了研究高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)HuN4株在其致弱过程中对仔猪外周免疫器官及肺脏损伤的变化,试验分别采用HuN4F5及其致弱代次F15、F23、F30及HuN4弱毒疫苗F112感染30日龄PRRSV抗原抗体阴性健康断奶仔猪,每天进行体温测定及临床症状观察,并在感染后0,3,5,7,10天检测病毒血症情况。病毒感染后10天剖杀试验猪,观察外周免疫器官及肺脏的病理变化并对肺脏和主要外周免疫器官病毒载量进行测定。结果表明:HuN4 F5、HuN4 F15组在临床症状、病毒血症、外周病毒载量及病理变化方面都明显比其他组严重,HuN4 F23组发病明显减轻,HuN4 F30组、HuN4 F112组无明显发病特征,与空白对照组无明显差异。说明HP-PRRSV HuN4株在致弱过程中,传代至30代时毒力发生显著减弱。 To study the lesion changes in peripheral immune organs and lung of the piglets infected with HP - PRRSV HuN4 in the course of its attenuation, the HuN4 strain (F5), its attenuated generations in F15, F23, F30, and the attenuated vaccine HuN4 F112 were used to infect the 30 - day - old healthy pigs tested negative for PRRSV antigen/antibody. All measurements of the body temperature were carried out and the clinical symptoms were observed every day, the HuN4 viremia was detected on days of 0, 3, 5,7,10 days after infection. The experimental pig- lets were sacrificed at 10 days after the virus infection, and then the pathological changes in peripheral immune organs and lung of the piglets were observed, and the viral loads of the major peripheral immune organs were measured. The results showed that the piglets of the HuN4 F5 and HuN4 F15 groups, were significantly wo^e than those of the other groups in terms of the clinical symptoms, viremia, viral loads and patho- logical changes in peripheral immune organs and lung. The clinical symptoms in the HuN4 F23 group were significantly reduced, and there were no obvious clinical symptoms between the HuN4 F30 and HuN4 F112 groups, which was no significant difference compare with the blank con- trol group. The results indicate when the HP - PRRSV HuN4 strain in the course of its attenuation was passaged for 30 generations, the viru- lence of the HuN4 F30 strain is significantly decreased.
出处 《黑龙江畜牧兽医》 CAS 北大核心 2014年第5期4-6,10,226,共5页 Heilongjiang Animal Science And veterinary Medicine
基金 "十二五"高技术研究发展计划项目(2011AA10A213) 黑龙江省科技公关项目(GA09B302)
关键词 高致病性猪繁殖与呼吸综合征病毒 HuN4株 致弱毒株 外周免疫器官 肺脏 病理损伤 HP- PRRSV HUN4 attenuated strain peripheral immune organs lung pathological damage
  • 相关文献

参考文献10

  • 1MEULENBERG J J, HULST M M, de MEIJER E J, et al. Lelystad virus, the causative agent of porcine epidemic abortion and respira- tory syndrome ( PEARS ), is related to LDV and EAV[J]. Virolo- gy, 1993, 192(1) : 62 -72.
  • 2郭宝清,陈章水,刘文兴,崔益洙.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996(2):1-5. 被引量:923
  • 3LENG X, LI Z, XIA M, et al. Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus po- tentially related to attenuation [ J ]. Vet Microbiol, 2012,157 ( 1/2 ) : 50 - 60.
  • 4LIU Y G, SH1 W D, ZHOU E M, et al. Dynamic changes in inflam-of blood and tureen fluid by gas chromatography [ J ] J Dairy Sei, 1961,44(9) :1768 - 1771.
  • 5何玉利,刘永刚,王刚,石文达,武嘉男,刘鹤,董建国,韩梓峰,姜成刚,田志军,蔡雪辉.不同毒力猪繁殖与呼吸综合征病毒对仔猪肺和外周免疫器官损伤的研究[J].中国预防兽医学报,2012,34(1):6-10. 被引量:6
  • 6韦天超,田志军,安同庆,周艳君,肖燕,姜一峰,郝晓芳,张善瑞,彭金美,仇华吉,童光志.猪繁殖与呼吸综合征病毒Taq Man-MGB荧光定量RT-PCR方法的建立和应用[J].中国预防兽医学报,2008,30(12):944-948. 被引量:29
  • 7邓治邦,潘荞,袁安文,王乃东,薛立群.副猪嗜血杆菌血清5型岳阳株对昆明小鼠淋巴器官损伤的研究[J].中国预防兽医学报,2011,33(1):41-44. 被引量:3
  • 8TIAN Z J, AN T Q, ZHOU Y J, et al. An attenuated live vaccine based on highly pathogenic porcine reproductive and respiratory syn- drome virus ( HP - PRRSV) protects piglets against HP - PRRS [ J 1. Vet Mierobio1,2009,138 (1/2) : 34 - 40.
  • 9AN T Q,TIAN Z J,ZHOU Y J,et al. Comparative genornic analysis of five pairs of virulent parental/attenuated vaccine strains of PRRSV [ J ]. Vet Microbiol,2011,149 ( 1/2 ) : 104 - 112.
  • 10周艳君,田志军,于海,等.高致病性猪繁殖与呼吸综合征病毒HuN4株及其致弱过程中不同代次病毒的遗传变异分析[c]//中国畜牧兽医学会动物传染病学分会.中国畜牧兽医学会家畜传染病学分会第七届全国会员代表大会暨第十三次学术研讨会论文集(上册).南宁:中国畜牧兽医学会动物传染病学分会,2009:380.

二级参考文献26

  • 1郭宝清,陈章水,刘文兴,崔益洙.从疑似PRRS流产胎儿分离PRRSV的研究[J].中国畜禽传染病,1996(2):1-5. 被引量:923
  • 2尹秀凤,张书霞,王艳,姜平,汤景元.副猪嗜血杆菌人工感染猪的病理学观察[J].中国兽医学报,2007,27(1):91-94. 被引量:16
  • 3童光志,周艳君,郝晓芳,田志军,仇华吉,彭金美,安同庆.高致病性猪繁殖与呼吸综合征病毒的分离鉴定及其分子流行病学分析[J].中国预防兽医学报,2007,29(5):323-327. 被引量:390
  • 4Wensvoort G, Terpstra C, Pol JMA, et al. Mystery swine disease in the Netherlands: the isolation of Lelystad virus [J]. Vet Q, 1991, 13(3): 121-130.
  • 5Collins J, Benfield D A, Christianson W T, et al. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs [J]. J Vet Diagn Invest, 1992, 4: 117-126.
  • 6Tian, K, Yu X, Zhao T, et al. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark [J]. PLoS ONE, 2007, 2(6): 1-10.
  • 7Wasilk A, Callahan J D, Christopher-Hennings J, et al. Detection of U S, Lelystad, and European-Like porcine reproductive and respiratory syndrome viruses and relative quantitation in boar semen and serum samples by real-time PCR [J]. Journal of Clinical Microbiology, 2004, 42(10): 4453-4461
  • 8Chung W B, Chan W H, Chaung H C, et al. Real-time PCR for quantitation of porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 in naturally-infected and challenged pigs [J]. Journal of Virological Methods, 2005, 124 (1-2): 11-19
  • 9Wills R W, Doster A R, Galeota J A, et al. Duration of infection and proportion of pigs persistently infected with porcine reproductive and respiratory syndrome virus [J]. Journal of Clinical Microbiology, 41(1): 58-62
  • 10Lew A E, Bock R E, Molloy J B, et al. Sensitive and specific detection of proviral bovine leukemia virus by 5' Taq nuclease PCR using a 3' minor groove binder fluorogenic probe [J]. J Virol Methods, 2004, 115(2): 167-175.

共引文献947

同被引文献14

  • 1Hopper S A, White M E, Twiddy N. An outbreak of blue-eared pig disease (porcine reproductive and respiratory syndrome) in four pig herds in Great Britain [J].Vet Record, 1992, 131(7): 140-144.
  • 2Albina E. Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview [J].Vet Microbiol, 1997, 55(1-4): 309-316.
  • 3Blaha T. The "colorful" epidemiology of PRRS [J].Vet Res, 2000, 31(1): 77-83.
  • 4Tian Ke-gong, Yu Xiu-ling, Zhao Tie-zhu, et al. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark [J].PLoS One, 2007, 2(6): e526.
  • 5Zhou Yan-jun, Hao Xiao-fang, Tian Zhi-Jun, et al. Highly virulent porcine reproductive and respiratory syndrome virus emerged in China [J].Transbound Emerg Dis, 2008, 55(3-4): 152-164.
  • 6Wang Yue, Liang Ya-jie, Han Jun, et al. Attenuation of porcine reproductive and respiratory syndrome virus strain MN184 using chimeric construction with vaccine sequence [J].Virology, 2008, 371(2): 418-429.
  • 7Kwon B, Ansari I H, Pattnaik A K, et al. Identification of virulence determinants of porcine reproductive and respiratory syndrome virus through construction of chimeric clones [J].Virology, 2008, 380(2): 371-378.
  • 8Zhou Lei, Zhang Jia-long, Zeng Jing-wen, et al. The 30-amino- acid deletion in the Nsp2 of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China is not related to its virulence [J].J Virol, 2009, 83(10): 5156-5167.
  • 9Li Yan, Zhou Lei, Zhang Jia-long, et al. Nsp9 and Nsp10 contribute to the fatal virulence of highly pathogenic porcine reproductive and respiratory syndrome virus emerging in China [J].PLoS Pathog, 2014, 10(7): e1004216.
  • 10Ma Jing-jiao, Shen Hui-gang, Liu Qin-fang, et al. Pathogenicity and transmissibility of novel reassortant H3N2 influenza viruses with 2009 pandemic H1N1 genes in pigs [J].J Virol, 2015, 89(5): 2831-2841.

引证文献1

二级引证文献1

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部