摘要
利用紫外线诱变育种方法分离对前期分离到一株葡聚糖酶野生菌株P5进行诱变。通过平板初筛、摇瓶复筛,从诱变菌株中筛选出一株高产菌株,其发酵酶活力可以达到5.85U/mL;在此基础上,对诱变菌株的发酵产酶工艺进行了初步的优化,其最适碳源为淀粉,最适氮源为花生饼粉,正交试验结果表明,淀粉含量为5%、花生饼粉含量为4%、NaH2PO4含量为0.2%时,该菌株的产酶量最高,酶活力达到21.23U/mL。
UV mutation breeding method was used to mutate a glucanase-producing wild strain P5 isolated in earlier stage. A high glucanase-producing strain was screened by plate screening and shaking flask culture with glucanase activity of 5.85 U/ml. Furthermore, the fermentation medium of mutant strain was optimized, and the optimum carbon source and nitrogen source was starch and peanut meal, respectively. The optimum medium formula was determined as follows: soluble starch 5%, peanut meal 4%, NaH2PO4 0.2%. Under these conditions, the fermentation glucanase activity reached up to 21.23 U/ml.
出处
《中国酿造》
CAS
2014年第4期90-93,共4页
China Brewing
基金
2013年建设中医药强省科研项目(20131277)
广东食品药品职业学院自然科学青年基金项目(2009YZ005)
关键词
枯草芽孢杆菌
葡聚糖酶
酶活力
诱变
突变株
Bacillus subtilis
glucanase
enzyme activity
mutation
mutant strain