摘要
目的通过对宫颈病变中脆性组氨酸三联体(Fragile histidine traid,FHIT)基因上三个微卫星位点D3S1300、D3S1234和D3S1481的微卫星不稳定性(Microsatellite instability,MSI)分析,探讨其与宫颈癌的发生、发展的关系。方法采用聚合酶链反应-非变性聚丙烯酰胺凝胶电泳-溴化乙锭染色技术检测FHIT基因中3个微卫星位点D3S1300、D3S1234和D3S1481的MSI。结果 MSI在宫颈上皮内瘤样病变(Cervical intraepithelial neoplasia,CIN)组与宫颈癌组检出率差异有统计学意义。在宫颈癌中I期与II期差异无统计学意义,与III^IV期比较差异有统计学意义,MSI的发生率与临床分期有相关,与病理分级无关。结论 MSI在宫颈癌中的检出率远远高于CIN,表明MSI在宫颈癌发展过程中起重要作用,是宫颈癌恶性进展中的早期事件。
Objective To investigate the instability of three microsatellite loci D3S1300, D3S1234 and D3S1481 on cervical lesions of fragile hisfidine triplet （fragile histidine traid, FHIT） gene and explore its relationship with the occurrence and development of cervical cancer. Methods Polymerase chain reaction- non-denaturing polyacrylamide gel electrophoresis - ethidium bromide staining were used to detect instability of three mierosatellite loci D3S1300, D3S1234 and D3S1481on FHIT gene. Results MSI in CIN and cervical cancer detection rate significandy. In cervical cancer stage I and stage II the difference was not statistically significant, and stage III - IV difference was statistically significant, MSI incidence associated with the clinical stage, histological grade has nothing to do with. Conclusions MSI in cervical cancer detection rate is much higher than in CIN, MSI in cervical cancer development process that plays an important role in the malignant progression of cervical cancer in the early events.
出处
《中国热带医学》
CAS
2014年第3期320-322,共3页
China Tropical Medicine
基金
邯郸市科技局科技计划项目(No.1323108087-13)
