摘要
在生理条件下,用荧光光谱法研究了氯诺昔康对牛血清白蛋白,铜(Ⅱ)对牛血清白蛋白以及铜(Ⅱ)对氯诺昔康和牛血清白蛋白荧光光谱特性的影响。铜(Ⅱ)和氯诺昔康均可使牛血清白蛋白的荧光强度发生猝灭,在铜(Ⅱ)存在下,氯诺昔康对牛血清白蛋白的荧光猝灭作用显著增强。根据荧光猝灭双倒数图计算氯诺昔康和牛血清白蛋白之间的结合常数是5.43×105,结合位点数是1.14。铜(Ⅱ)和牛血清白蛋白之间的结合常数是2.10×105,结合位点数是0.78。
Under the physiological conditions, fluorescence spectrometry was used to study bovine serum albumin, copper （ Ⅱ ） of bovine serum albumin and copper （ Ⅱ ） on bovine serum albumin fluorescence spectrum characteristics. Copper （ Ⅱ ） and lornoxicam could both cause bovine serum albumin fluorescence quenched. Under the existence of copper （ Ⅱ ）, lornoxicam enhanced the fluorescence quenching effect of bovine serum albumin. According to lineweaver-burk plot calculation, the combining constant between lornoxicam and bovine serum albumin was 5.43＆#215;105, position points were 1.14. The combining constant between Copper （Ⅱ） and bovine serum albumin was 2.10＆#215;105, position points were 0.78.
出处
《化工技术与开发》
CAS
2014年第4期15-18,共4页
Technology & Development of Chemical Industry
基金
教育部"春晖计划"基金资助项目(2009-1-01040)
内蒙古教育厅高校科研基金项目(NJZY11145)
内蒙古科技大学创新基金资助项目(2012NCL034)
内蒙古科技大学大学生创新基金项目
