摘要
目的对2009—2012年甘肃省流感监测结果进行分析，为甘肃省的流感防控工作提供科学依据。方法全省19家国家级流感监测哨点医院采集流感样病例的咽拭子，各地医疗机构送检流感样暴发疫情标本，以荧光定量PCR方法检测病毒核酸，用MDCK进行病毒分离培养，送中国疾病预防控制中心病毒病所进行复核及序列分析。结果共采集流感样病例咽拭子36892份，检测出流感核酸阳性8030例，检出率为21．77％，其中新甲型H1N14331份，季节性H31562份，季节性H196份，B型1651份，A未分型380份。对7073份标本进行病毒分离，分离到病毒1149株，其中新甲型H1N1346株，季节性H3183株，季节性H134株，B型Victorian系470株，Yamagata系116株。按年度分析，2009—2012年流感病毒检出率依次为32．71％、13．95％、7．80％和19．64％；按月分析，在10-12月份A型流感病毒阳性率及构成比均高于其他月份，阳性率平均为31．33％，构成比为97．61％；2—4月份B型流感病毒阳性率及构成比分别为16．51％和81．10％；5—9月份为流感病毒阳性率仅为5．03％（2009年不统计）。按年龄组分析，〈1岁婴儿检出率最低为7．21％，显著低于与其他年龄组；10—20岁最高为34．26％。结论甘肃省2009年以新甲型H1N1流感流行为主，2010—2011年流感活动很弱，仅为散发，2012年春季以B型为主，11月开始以甲型H1N1及季节性H3为主。流感流行每年均有一个高峰，病毒型别表现为交替流行的规律，甲型流感是冬季儿童常见的传染病。
Objective To study the epidemical characteristics of influenza in Gansu province from 2009 to 2012, and to discuss the changes of the predominant strains of influenza virus. In order to provide scientific basis for defending influenza effectively. Methods Influenza surveillance was performed by collecting the pharyngeal swab specimens from influenza patients of national surveillance hospitals and places with suspected influenza epidemic situations, inoculated on MDCK cell culture to obtain the influenza virus strains. Results From Jan. 2009 to Dce. 2012 36892 specimens were collected, and 8030 were positive for influenza virus, including 4331 influenza A （H1N1）, seasonal H3N2 subtype1562, H1N1 subtype 96, 1651 type B, and 380 type A with unknown subtype strains. During the period from 2009 to 2012, the positive of influenza virus isolation was 32.71% , 13.95% , 7.80% , and 19.64% , respectively. Totally 1149 influenza virus strains were isolated,positive rate was 16.24% , of them, 346 strains were SWA1 （HlpdmN1） ,34 strains were A1 （H1N1）, 183 strains were A3 （H3N2）, 470 strains were subtype B of Victoria and 116 strains were subtype B of Yamagata. Conclusion In 2009 new influenza A （H1N1） was the major epidemic, and in 2010-2011 the activity of influenza was very weak, only presented as sporadic disease. In the spring of 2012, type B influenza was dominant, while since Nov. type A （ H1N1 ） and seasonal H3 were dominant. There was a peak of influenza epidemic each year, and alternative viral types predominance was the rule.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2014年第2期120-122,共3页
Chinese Journal of Experimental and Clinical Virology
关键词
流感病毒A型
流感病毒B型
免疫学监测
聚合酶链反应
病毒培养
Influenza A virus
Influenza B virus
Immundogic surveillance
Polyomerage chainreaelion
Virus cultirations
