摘要
目的建立一种灵敏、快速、专属性好、选择性强的HPLC-MS/MS分析法用于测定Beagle犬血浆样品中盐酸莫西沙星的含量,研究其药动学。方法用HPLC-MS/MS法测定血浆中盐酸莫西沙星的含量;以环丙沙星为内标,色谱柱为XTerra@MS C18 5μm,2.1 mm×150 mm分析柱,流动相为甲醇-水(50∶50,v/v),水相中含0.1%甲酸;流速为0.2 mL/min,进样10μL。检测离子对分别为:莫西沙星m/z 402.1→m/z 384.3,内标环丙沙星m/z 332.2→m/z314.2。结果莫西沙星在10μg/L^2 000μg/L的范围内线性良好,最低定量限10μg/L,准确度在87.8%~111.6%之间,日内、日间精密度(RSD)均≤11.6%,提取回收率>80%。犬口服给药,血浆中盐酸莫西沙星药时曲线呈两室模型,药动学参数t1/2、tmax、AUC0-t、AUC0-∞、CL分别为10.7 h、2.1 h、3 943μg/L·h、4 177μg/L·h和10.5 L/h/kg。结论分析方法操作简便、专属性强、灵敏度高,适用于Beagle犬血浆中盐酸莫西沙星含量测定及药动学研究。
Objective To establish a sensitive and specific LC-MS/MS method for the determination of moxifloxacin hydrochloride in Beagle dog plasma and to evaluate the pharmacokinetic profile.Methods The concentration of Moxifloxacin Hydrochloride was determined by LC-MS/MS with XTerra@MS C18 column （5 μm,2.1 mm ×150 mm）.The mobile phase consisted of methanol-water （50∶ 50,v/v） with the flow rate of 0.2 mL/min.The injection volume was 10 μL.Ions of moxifloxacin and ciprofloxacin were m/z 402.1→m/z 384.3 （F =120 V,CE =25）,m/z 332.2→m/z 314.2（135 V,CE =20）.Results Standard calibration graph for moxifloxacin hydrochloride was linear over a curve range of 10 μg/L ～2 000 μg/L（R2 ＞0.99） and the low limit of quantification was 10 μg/L using a plasma sample of 100 μL.The intra-day and inter-day precision （RSD） were in line with analysis of the biological samples.RSD were less than 11.6％,the extraction recoveries were greater than 80％.The concentration-time curves of moxifloxacin hydrochloride were best fitted to a two compartment model.The main pharmacokinetic parameters of t1/2,tmax,AUC0-t,AUC0-∞ and CL were 10.7 h,2.1 h,3 943 μg/L · h,4 177 μg/L · h and 10.5 L/h · kg respectively.Conclusions The method was specific and suitable for the determination of moxifloxacin hydrochloride in Beagle dog plasma and to study the pharmacokinetic character.
出处
《基础医学与临床》
CSCD
北大核心
2014年第5期684-689,共6页
Basic and Clinical Medicine
