期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

补体C5a对低氧脑损伤大鼠分泌IL-6的调节作用研究

Research of the regulating effect of complement C5a on the secretion of IL-6 in rats with hypoxic brain injury
下载PDF
导出
摘要 目的观察大鼠低氧脑损伤环境下补体C5a对大脑皮质细胞分泌IL-6的调节作用。方法选用成年体质量均等的雄性大鼠54只,分为1%低氧建模组(A组)18只、4%低氧建模组(B组)18只和常氧量对照组(C组)18只。各组在相应环境下处理3 h后,采用real-time PCR技术,检测建模大鼠给予抗C5a单克隆抗体以及外源性C5a后IL-6分泌水平。结果 A、B组较C组补体C5a水平明显升高(P均<0.05),IL-6水平降低。给予等量抗C5a单克隆抗体后,A组IL-6明显低于B、C组。给予外源性C5a后,A组IL-6水平明显高于B、C组。结论补体C5a在大鼠低氧脑损伤时对IL-6分泌有调节作用。 Objective It is to observe the regulating effect of complement C5a on the secretion of IL-6 by cerebral cortical cells in rats with hypoxic brain injury.Methods 54 adult male rats with equal weight were divided into 1% hypoxic model group,4% hypoxic model group and normal oxygen control group,each group had 18 rats.The level of IL-6 was detected by real-time PCR technology in the rats after modeling under corresponding conditions and giving anti-C5a monoclonal antibody and exogenous C5a.Results The levels of complement C5a were significantly higher and the level of IL-6 was lower in group A and group B than that in group C （P 〈 0.05）.After giving anti-C5a monoclonal antibody,the level of IL-6 in group A was lower than that of group B and C.After giving exogenous C5a,the level of group A was higher than that in group B and C.Conclusion Complement C5a has regulation effect on IL-6 secretion in rats with hypoxic brain injury.
作者 周亚妮 李可
机构地区 西安交通大学医学院 商洛职业技术学院
出处 《现代中西医结合杂志》 CAS 2014年第14期1498-1499,共2页 Modern Journal of Integrated Traditional Chinese and Western Medicine
基金 陕西省教育科学规划课题(SGH13557)
关键词 补体C5A 重度低氧脑损伤 IL-6 大鼠 complement C5a severe hypoxic brain injury IL-6 rat
分类号 R-332 [医药卫生]
  • 相关文献

参考文献10

  • 1胡强,陈高.缺氧诱导因子-1α在缺血缺氧脑损伤中的作用研究进展[J].国际神经病学神经外科学杂志,2013,40(3):258-262. 被引量：11
  • 2Di li, Maccarone R, Corvetti L, et al. Differential modulation of in- terleukin- 6 expression by interleukin - 113 in neuronal and glial cultures [ J ]. Cytokine Netw,2003,14 ( 9 ) :97 - 102.
  • 3Loddick SA,Turnbull AV, Rothwell NJ. Cerebral interleukin - 6 is neuroprotective during permannent focal cerebral ischemia in the rat [ J]. Cereb Blood Flow Metabol, 1998,18 ( 11 ) : 176 - 179.
  • 4Gorina R, Petegnief V, Chamorro A, et al. AG490 prevents cell death afent exposure of rat astrocyte to hydrogen peroxide or proin- flammatory cytokines : involvement of the jak2/STAT pathway [ J ]. J Neurochem, 2005,92 ( 6 ) : 505 - 518.
  • 5Wang H,Duan X, Ren Y, et al. FoxO3a negatively regulates nerve growth factor-induced neuronal differentiation though inhibiting the expression of neurochondrin in PC12 cells [ J]. Mol Neurobiol, 2013,47(4) :24 - 36.
  • 6李华华,娄淑杰.低氧对大鼠大脑皮层细胞表达IL-1β、IL-6和TNF-α mRNA的影响[J].神经解剖学杂志,2013,29(2):141-144. 被引量：5
  • 7Liu X, He L, Stensaas L, et al. Adaptation to chronic hypoxia in- volves immune cell invasion and increased expression of inflammato- ry cytokines in rat carotid body[ J]. Physiol Lung Cell Mol Physiol, 2009,296(21 ) :L158 - L166.
  • 8Li S, Qian XH,Zhou W,et al. Time-dependent inflammatory factor production and NFKB activation in a rodent model of intermittent hy- poxia[J]. Med Sci,2011,141( w13309):1-11.
  • 9肖燕,谢晓伟,龚琴,文仲光.小鼠哮喘模型中补体C5a对IL-17表达的调节作用[J].临床肺科杂志,2012,17(8):1361-1363. 被引量：4
  • 10Ke Li, Henrieta Fazekasova, et al. Function of human monocytes de- rived DCs by anaphylatoxins C3a and CSa [ J]. Immunobiology, 2012,217(6) :65 -73.

二级参考文献36

  • 1Zhao Y, Yang J, Gao YD. Altered Expressions of Helper T Cell (Th) 1, Th2, and Th17 Cytokines in CD8( + ) and ~/8 T Cells in Patients with Allergic Asthma[ J ]. J Asthma, 2011,48 ( 5 ) :429 - 36.
  • 2Lambrecht BN. An unexpected role for the anaphylatoxin C5a re- ceptor in allergic sensitization [ J ]. J Clin Invest,2006,116 ( 3 ) :628 -32.
  • 3Kohl J, Baelder R, Lewkowich IP, et al. A regulatory role for the CSa anaphylatoxin in type 2 immunity in asthma[J]. J Clin Invest, 2006,116(3) :783 -96.
  • 4Hawlisch H, Wills-Karp M, Karp CL,et al. The anaphylatoxins bridge innate and adaptive immune responses in allergic asthma [ J ]. Mol Immuno1,2004,41 (2 - 3 ) : 123.
  • 5Lajoie S, Lewkowieh IP, Suzuki Y, et al. Complement-mediated regulation of the IL-17A axis is a central genetic determinant of the severity of experimental allergic asthma[ J]. Nat Immunol, 2010, 11 (10) :928 -35.
  • 6Zhang X, Schmudde I, Laumonnier Y, et al. A critical role for C5L2 in the pathogenesis of experimental allergic asthma[ J]. J Im- muno1,2010,185 ( 11 ) :6741 - 52.
  • 7Chen Y, Thai P, Zhao YH, et al. Stimulation of airway mucin gene expression by interleukin IL-17 through IL-6 paraerine_autocrine loop [ J ]. J Biol Chem ,2003,278 : 17036 - 17043.
  • 8Herrick CA, Bottomly K. To respond or not to respond: T cells in allergic asthma. Nature review[J]. Immunol,2003,3:405 -412.
  • 9Wang CK, Ho CY, Ko FW, et al. Preinflammatory cytokines ( IL- 17, IL-6, IL-18 and IL-12) and Th cytokines( IFN-g,IL-4, IL-10 and IL-13) in patients with allergic asthma[J]. Clin Exp Immunol, 2001,125 : 177 - 183.
  • 10Bullens DM, Truyen E, Coteur L,et al. IL-17 mRNA in sputum of asthmatic patients: linking T cell driven inflammation and granulo- cytic influx [ J ] ? Respir Res, 2006,7 : 135.

共引文献17

现代中西医结合杂志
2014年 第14期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部