摘要
目的观察慢性氟中毒大鼠脊髓白质的损伤及其机制及短期脱氟治疗后脊髓白质损害的恢复情况。方法120只Wistar大鼠随机分为4组，高氟组饮氟化水（200mg／L）；高氟对照组饮蒸馏水；脱氟组先饮氟化水（200mg／L），12周后改为蒸馏水；脱氟对照组饮蒸馏水，实验期24周。分别于第4、8和12周测定4组大鼠尿氟含量，于第16、20、24周测定脱氟组和脱氟对照组尿氟含量，并在上述各个时间点应用BBB的评估标准及斜板试验分别对大鼠的脊髓功能进行行为学评估。12周时将高氟组和高氟对照组大鼠处死。脱氟组改饮蒸馏水，24周时将脱氟组及脱氟对照组大鼠处死，所有大鼠均取颈脊髓备电镜观察；取上胸髓行免疫组织化学染色观察MBP的表达；取下胸髓行免疫印迹检测MBP蛋白的含量。结果大鼠饮高氟水8周后，高氟组大鼠BBB评分及斜板试验结果显著低于对照组[BBB评分（19．8±4．1）分比（17．0±3．9）分]；斜板试验[（56．2±4．2）。比（60．1±4．0）°，P〈0．01]，脱氟组与高氟组相比未见明显改善；电镜检查发现高氟组大鼠脊髓白质呈慢性损害病理改变，脱氟组大鼠脊髓已经发生的慢性损害未见恢复；高氟组大鼠脊髓组织中MBP与高氟对照组比较表达显著降低；而脱氟组大鼠与高氟组MBP表达差异无统计学意义。结论慢性氟中毒可以造成大鼠脊髓功能的损伤，与氟中毒造成脊髓白质脱髓鞘有关，短时间脱氟治疗后，该损伤不能恢复。
Objective To explore the injury mechanism for white matter of spinal cord and the improvement of function after defluoriation. Methods A total of 120 Wistar rats were separated randomly into 4 groups （ n = 30 each）. High floufiod group received high concentration NaF water （ 200 mg/L） to establish fluorosis model; control group distilled water; deflourination group high concentration NaF water （200 mg/L） for 12 weeks and then distilled water for 12 weeks; defluorination control group. The urinary contents of fluoride were detected at Weeks 4, 8 and 12. The first two groups were sacrificed at Week 12 while the other two groups at Week 24. The spinal cord functions were detected by BBB scale and incline plate test. Their cervical spinal cord tissues were collected and observed under electron microscope. The expression of myelin basic protein （MBP） in thoracic cord was detected by immunohistochemistry and Western blot. The comparison of measurement data was performed with 17 test and correlation analysis. Cytological changes of white matter in spinal cord were detected after chronic fluorosis. Results The spinal functions of high flouriod and deflourination groups were inferior to those of the control groups. But no difference existed among the groups. Pathological manifestations of chronic white matter injury of spinal cord could be found in high flouriod and deflourination groups. The MBP expression in spinal cord of fluorosis and deflurination groups decreased in comparison with those in control groups. But no difference existed among them. Conclusion White matter injury of spinal cord is present in chronic fluorosis rats. Defluoriation for a short time offers no recovery.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2014年第15期1189-1192,共4页
National Medical Journal of China
基金
天津市卫生局科技基金（09KY22）
关键词
脊髓损伤
氟
中毒
Spinal cord injuries
Fluorine
Poisoning
