摘要
目的观察Fascin_1在人类肺癌及癌旁组织中的表达及其与肺癌患者临床病理特征之间的关系,并研究Fascin_1表达与肺癌的临床生物学行为的关系。方法采用免疫组化MaxVision两步法检测84例肺癌及癌旁组织中Fascin_1蛋白的表达。用Fascin_1在血管内皮细胞中的表达作为阳性对照,使用PBS代替一抗作为阴性对照。结果 Fascin_1蛋白在84例肺癌组织及癌旁组织中分别为78例(92.9%)阳性与27例(32.1%)Fascin_1蛋白阳性,两者差异有统计学意义(P<0.05)。Fascin_1表达在不同年龄、临床分期以及区域淋巴结转移间差异有统计学意义(P<0.05),但是在不同性别、T分期以及病理类型间差异无统计学意义(P>0.05)。肺癌组织中Fascin_1蛋白阳性表达强度不同的患者的生存时间差异有统计学意义(P<0.05),而癌旁组织中Fascin_1蛋白阳性表达强度不同患者的生存时间差异无统计学意义(P>0.05)。结论在肺癌中,Fascin_1的表达同肿瘤的侵袭和转移密切相关,同时癌组织中Fascin_1的不同表达对患者的生存时间有明显影响。因此考虑Fascin_1有望成为肺癌潜在生物学标志物。
Objective To observe the relationship of Fascin_1 expression in human lung cancer and para-cancer tissues to clinicopathological characteristics in lung cancer patients, and to investigate the correlations of Fascin_1 expression with the biologic behaviors of lung cancer. Methods Immunohistochemistry MaxVision method was a-dopted to detect the protein expression of Fascin_1 in 84 cases of lung cancer and para-cancer tissues. Fascin_1 ex-pression in vascular endothelial cells was used as the positive criteria, while PBS displaced primary antibody as the negative criteria. Results The positive rates of Fascin_1 expression in lung cancer tissues and para-cancer tissues were 92. 9% （78/84） and 32. 1% （27/84） respectively, with significant difference（P〈0. 05）. Fascin_1 expres-sion was correlated with age, clinical stage and regional lymph node metastasis, with significant differnence（ P〈0.05）. Whereas it was not correlated with gender, T stage and pathology type, with no statistical difference（P〉0.05 ） . The overall survival time was significantly different among the different intensities of Fascin_1 expression in lung cancer tissues（P〈0. 05）, while there was no statistical difference among the different intensities of Fascin_1 expression in para-cancer tissues（P〉0. 05）. Conclusion Fascin_1 plays an important role in the invasiveness and metastasis of lung cancer. Fascin_1 expression in cancer tissues has an obvious influence on the overall survival time of patients. Fascin_1 might be potential candidate of biological biomarkers for lung cancer.
出处
《安徽医科大学学报》
CAS
北大核心
2014年第5期658-661,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81101597
81172533)
安徽高校省级科学研究(编号:KJ2012z142)
安徽医科大学校基金及追加基金(编号:2012xkj001zj)
