摘要
目的:测定加味丹参饮中丹参酮ⅡA和丹酚酸B的含量。方法:采用HPLC法,色谱柱:(4.6 mm×250 mm,5μm);丹参酮ⅡA:流动相为甲醇-水(75∶25);流速:1 mL·min-1;柱温:30℃;λ=270 nm;丹酚酸B:流动相:甲醇乙腈-甲酸-水(30∶10∶1∶59);流速:1 mL·min-1;柱温:30℃;λ=286 nm。进样量:10μL。结果:丹参酮ⅡA和丹酚酸B分别在0.016-0.32μg,0.14-2.8μg内呈良好的线性关系;平均回收率分别为101.2%(RSD%=2.47%,n=5),99.6%(RSD%=2.71%,n=6)。结论:该方法准确、可靠、重复性好,可用于加味丹参饮的质量控制。
Objective： To determine the content of Tanshinone IIA and SalVianolic Acid B in the Jiawei Danshen Decoction. Methods： The contents of Tanshinone IIA and SalVianolic Acid B in the Jiawei Danshen Decoction are determined by HPLC method. Chromatographic column （4.6 mm×250mm,5μm）, for Tanshinone IIA, mobile phase （Methanol-water 75：25）; flow rate 1 mL·min-1; column temperature 30 ℃ ;h=270 nm; for SalVianolic Acid B, mobile phase （Methanol acetonitrileformic acid - water 30：10：1：59）; flow rate 1 mL·min-1; column temperature 30℃;h=286 nm; sample size of 10μL. Results： The content of Tanshinone IIA and SalVianolic Acid B are respectively 0.016-0.32μg and 0.14-2.8 μg which showed well linear relation. The average recoveries （n=5） of Tanshinone IIA and SalVianolic Acid B were 101.2%（RSD%=2.47%,n=5） and 99.6% （RSD%=2.71%,n=6）, respectively. Conclusion：The method is accurate, reliable and well iterancy. This method can use to control the quality of Jiawei Danshen Decoction.
出处
《中医药导报》
2014年第4期55-57,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(30973750)
湖南省教育厅资助项目(09A07I)
关键词
加味丹参饮
丹参酮ⅡA
丹酚酸B
HPLC
含量测定
Jiawei Danshen Decoction
Tanshinone IIA
SalVianolic Acid B
HPLC
Content determination
