摘要
该文建立了蒸制三七中人参皂苷Rgs，F4，Rk3，Rh4，20（S）-Rg3，20（R）-Rg3，Rkl，Rgs，20（S）-Rh2，20（R）-Rh：10种皂苷类活性成分同时含量测定的超高效液相色谱方法，并对其不同头数、不同药用部位、不同蒸制时间的样品进行含量测定和质量控制研究。所采用的色谱条件为ACQUITY BEH C18色谱柱（2．1mm×100mm，1．7Ixm），流动相乙腈-水，梯度洗脱，流速0．3mL·min^-1，检测波长203nm，柱温35℃。结果显示，人参皂苷Rg6，F4，Rk3，Rh4，20（S）-Rg3，20（R）-Rg3，Rk1，Rg5，20（S）-Rh2，20（R）-Rh2分另0在0．46—115，2．06—515，1．632～408，3．216～804，1．392—348，1．4～350，0．496～248，3．012～1506，0．82—205，0．832～208mg·L^-1具有良好的线性关系（R。≥0．9998），平均回收率分别为97．00％，97．96％，98．86％，95．27％，98．67％，98．02％，95．53％，96．63％，99．57％，103．6％。该方法快速、准确、重复性好、分离度高，可有效控制蒸制三七的质量，为制定其规范化炮制工艺和质量标准，确保其临床疗效的可靠性与-致性奠定了基础。
A quantitative method using ultrahigh-performance liquid chromatography was established to simultaneously determine ten ginsenoside active ingredients including ginsenoside Rg6, F4, Rk3, Rh4, 20 （S）-Rg3, 20 （R）-Rg3, Rk1, Rg5, 20 （S）-Rh2 and 20 （R）-Rh2 in steamed notoginseng. The ten ginsenosides of steamed notoginseng with different head numbers, parts, and steaming time were determined by this method. An Acquity BEH Cls chromatographic column （2. 1 mm ×100 mm, 1.7 μm） was used to perform the determination, which was maintained at 35 ℃ throughout the analysis. Mobile phase was composed of water and acetonitrile with flow rate at 0.3 mL · min^-1 under gradient elution, and detection wavelength was set to 203 nm for monitoring the separation. The resuits demonstrate ginsenoside Rg6, F4, Rk3, Rh4, 20 （S） -Rg3, 20 （R） -Rg3, Rk1, Rg5, 20 （S） -Rh2 and 20 （R） -Rh2 have shown good linearity （R2 i〉0. 999 8） within 0. 46-115, 2.06-515, 1. 632-408, 3. 216-804, 1. 392-348, 1.4-350, 0. 496-248, 3. 012-1 506, 0. 82-205 and 0. 832-208 mg · L^- 1, and their average recoveries were 97.00%, 97. 96%, 98.86%, 95.27%, 98.67% , 98.02% , 95.53%, 96. 63%, 99. 57% and 103.6%, respectively. The proposed approach was quick and accurate and portrayed excellent repeatability and determination efficiency. The quality of steamed notoginseng was effectively controlled, which served as a foundation for establishing a normalized processing technique and quality standard for ensuring the reliability and consistency of its clinical efficacy.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2014年第9期1614-1619,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金青年基金项目(81102812)
关键词
蒸制三七
人参皂苷
UPLC
多成分同时测定
质量控制
steamed notoginseng
ginsenoside
ultrahigh-performance liquid chromatography
simultaneous multicomponent determination
quality control
