摘要
采用研磨组织稀释分离法分离烟草植株中的内生细菌,对云南省不同品种、不同种植地区、不同组织器官、不同生长期的烟草内生细菌的菌群密度进行研究.结果表明,烟株的根、茎、叶中均存在大量的内生细菌,烟草内生细菌的分布存在品种差异、生长期差异、组织部位差异.有的内生细菌为常住菌群,有的为暂居菌群.在整个生育期中,以成熟期的内生细菌的数量最多.内生细菌的数量在云烟85、KRK26、K326、红花大金元植株不同器官中的分布规律依次为：根＞叶＞茎,叶＞根＞茎,叶＞根＞茎,根＞叶＞茎.4个品种烟草生长期总带菌量平均值波动幅度为2.6 ～4.96 log cfu/g.fw.种植在不同地区的同一烟草品种,内生细菌数量不尽相同.在分离到的所有内生细菌中以Baccillus属细菌分布最广,巳鉴定出8个种,包括Bacillm pumilm,B.amyloliquefaciens,B.licheniformis,B.subtilis,B.megaterium,B.flexus,B.simplx,B.cereus.对烟草这一重要经济作物内生细菌菌群密度动态变化进行研究,可为科学认知和利用这类微生物资源奠定基础.
In the study,some bacteria strains were isolated from different tissues of tobacco collected from different regions in Yunnan by the method of grinding separation.The population density of endophytic bacteria in different cultivars and tissues of tobacco at different growth stages in different planting areas were studied.The results showed that there were a lot of endophytic bacteria in root,stem and leaf of the tobacco plants.The population density of endophytic bacteria varied with different cultivars,different tissues,different growth stages and different planting areas.The distrbution of endophytic bacteria had season preference,tissue specificity and growth-stage heterogeneity.Some of them were resident flora,others were transient flora.The amounts of endophytic bacteria in maturing stage were most.The distribution laws of the bacteria populstion density in Yunyan85,KRK26,K326,Hongda were roots ＞ leaves ＞ stems,leaves ＞ roots ＞ stems,leaves ＞roots ＞ stems,roots ＞ leaves ＞ stems.The germ carrying amounts in four growth stages were in alternative changing process with the fluctuation amplitude of germ amount of 2.6-4.96 log cfu/g.fw.The endophytic bacteria with a higher frequency in tobacco were Bacillus sp.,it include Bacillus pumilus,B.amyloliquefaciens,B.licheriformis,B.subtilis,B.megaterium,B.flexus,B.simplex,Bacillus cereus.They were therefore considered as the dominant genera of endophytic bacteria in tobacco.The population density of endophytic bacteria varied with different areas.The studies on population dynamics could provide fundamental theory for biological control of tobacco insects and promoting growth.
出处
《西南农业学报》
CSCD
北大核心
2014年第2期682-687,共6页
Southwest China Journal of Agricultural Sciences
基金
云南省烟草公司科技项目(2012YN06)
昆明学院大学生科学研究项目(DXS14070)
关键词
烟草
内生细菌
菌群密度
分布特征
Tobacco
Endophytic bacteria
Bacteria population density
Distribution characteristics
