摘要
目的:探讨水通道蛋白-1(AQP-1)在急性坏死性胰腺炎伴高三酰甘油血症大鼠肠屏障功能障碍发病机制中的作用及山楂承气汤对其表达的影响。方法:雄性SD大鼠为研究对象,随机分为对照组、急性坏死性胰腺炎伴高三酰甘油血症(HANP)组、辛伐他汀联合奥曲肽(HX)组和山楂承气汤(HS)组,碘-淀粉酶比色法测定血清淀粉酶含量,ELISA法测定血清三酰甘油、游离脂肪酸、AQP-1及肿瘤坏死因子-α浓度,并用HE染色法观察小肠组织病理改变,EB血管外渗法检测小肠组织毛细血管通透性,FQ-PCR法测定小肠组织AQP-1蛋白及Western Blot法测定AQP-1 mRNA表达。结果:HANP组、HX组及HS组血清淀粉酶、三酰甘油、游离脂肪酸及肿瘤坏死因子-α水平显著高于对照组(P<0.05);HS组及HX组与HANP组相比显著降低(P<0.05),HX组较HS组显著下降(P<0.05)。HANP组、HX组及HS组AQP-1蛋白及mRNA含量显著低于对照组(P<0.05);HS组及HX组较HANP组显著升高(P<0.05)。HS组及HX组小肠组织病理改变较HANP组减轻;与HANP组相比,HX组与HS组小肠组织毛细血管通透性均明显降低(P<0.05)。结论:山楂承气汤能够明显改善模型大鼠生化及病理损伤,对毛细血管通透性增高引起的毛细血管渗漏亦有一定的抑制作用,其机制可能是通过调控AQP-1的表达来实现的。
Objective： To study the role of aquaporin-1 in intestinal endothelial barrier dysfunction of acute necrotizing pancreatitis with hypertriglyceridemia rats and to observe the influence of Shanzha Chengqi Decoction on its expression. Methods： Male SD rats were randomly divided into four groups： a control group, acute necrotizing pancreatitis with hypertdglyceridemia group （HANP group）, Simvastatin combined the ktreotide group （HX group） and Shanzha Chengqi Decoction group （HS group）. The serum amylase content was determined by using Iodine-amylase colorimetric method, ELISA method was used to determine the concentration of triacylglycerol （TG）, free fatty acids （FFA）, aquaporin-1 （AQP-1） and tumor necrosis factor alpha （TNF-ct）. HE staining method was used to observe intestinal tissue pathological changes, and EB vascular extravasation method was used to detect the blood capillary permeability of the intestinal tissue. FQ-PCR method and Weston Blot method were used to detect the expression of AQP-1 protein and mRNA in small intestinal tissue. Results： The Serum amylase, TG, FFA, TNF-ct level in HANP group, HX group and HS group were significantly higher than those in control group （P〈0.05）. HS group and HX group significantly reduced compared with HANP group （P〈0.05）, and they reduced more in HX group （P〈0.05）. The expression ofAQP-1 protein and mRNA in HANP group, HX group and HS group were significantly lower than those in control group （P〈 0.05）, and those in HS group and HX group significantly increased compared with HANP group （P〈0.05）. The intestine tissues pathological lesions of HS group and HX group were reduced than HANP group （P〈0.05）. Compared with HANP group, the blood capillary permeability of the intestinal tissue in HS and HX groups were significantly lower （P〈0.05）. Conclusions： Shanzha Chengqi Decoction could obviously improve the biochemical and pathological damage of model rats, which had a certain inhibition effect in capillary leakage resulted from the increase of capillary permeability. These effects might be carried out through the regulation of AQP-1 expression.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2014年第5期1612-1617,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
上海市教委创新项目(No.11YZ66)
上海市卫生局中医药科研基金(No.2010J011A)
上海市普陀区科委资助项目(No.2010PTKW006)
上海市教委预算内科研项目(No.200JW32)~~
