摘要
目的研究致婴幼儿败血症猪霍乱沙门菌的耐药基因特点及同源性。方法 16株猪霍乱沙门菌,采用K-B纸片扩散法检测抗菌药物的耐药性,同时用PCR法及DNA测序法对其进行β-内酰胺酶类耐药基因检测;PFGE法测定同源性。结果对氨苄西林耐药的13株猪霍乱沙门菌经PCR扩增后并测序为blaTEM-1,16株猪霍乱沙门菌经PFGE分型,可分为5个PFGE型,其中A型是最主要的克隆(12/16)。13株blaTEM-1基因阳性菌株分别为A型11株,B型1株,D型1株。结论产TEM-1型β-内酰胺酶是本地区猪霍乱沙门菌对氨苄西林耐药的主要机制,PFGE分型方法对猪霍乱沙门菌分型能力较好,克隆性传播为猪霍乱沙门菌的主要传播途径,同一PFGE型菌株的耐药谱非常接近。
Objective To investigate drug-resistant genes and homology of Salmonella choleraesuis isolates from the blood of children with septicemia. Methods A total of 16 strains of Salmonella choleraesuis were collected from children with septicemia. Antimicrobial susceptibility was determined by K-B disk,PCR and DNA sequencing were used for detecting β-1actamases and quinolones genes. Their homology was identified by PFGE. Results Thirteen strains of ampicillin resistant Salmonella choleraesuis were identified by PCR amplifying and DNA Sequencing and their drug-resistant gene yeas blaTEM-1. 16 strains of Salmonella cholcraesuis were classified into 9 PFGE types. A type was the most important clone （12/16）. Thirteen strains with blaTEU-I gene in- clude A type（11 strains）, B type（l strains） and D type（1 strains）. Conclusion For Salmonella choleraesuis, β-1actamases based on TEM-1 gene is the primary ampicillin resistant mechanism. PFGE is a proper method for Salmonella choleraesuis classification and the strains from same PFGE type have similar drug resistance spectrum. And clonal spread is the main transmission route for Salmonella cholemesuis.
出处
《实验与检验医学》
CAS
2014年第2期126-127,131,共3页
Experimental and Laboratory Medicine
基金
江西省卫生厅科技计划(编号:20133137)
关键词
猪霍乱沙门菌
耐药基因
同源性分析
Stdmonella choleraesuis
Resistance gene
Homology analysis
