摘要
构建3株表达凝乳酶的重组毕赤酵母，通过甲醇诱导收集BMMY液体培养基上清后，通过ArimaK方法分别对其进行酶活测试并进行酶学性质分析，结果表明，GS115／pPICgK-chyl菌株诱导7d后，其酶活达到1.905 SU/mL重组凝乳酶的最适反应温度为45℃；最适反应pH为6；存pH4-7之间凝乳活性相对稳定；在50℃以下可以保持酶活，在55℃、处理50min后，丧欠60％的活性．
Three recombinant Pichia pastoris expressing chymosin were constructed successfully in this experiment. Methanol was used to induce them, then culture supernatants BMMY were collected for further study. The enzyme activity was tested according to method of Arima K. The characterizations of chymosin from recombinant Pichia pastoris were also studied. Results showed that the optimal temperature and pH was 45 ℃ and 6.0 respectively. After GS115/pPIC9K chyl strain induced for 7 days, the enzyme activity of the supernatant reached 1. 905 SU/mL. The activity of recombinant chymosin was remained from pH 4. 0 to 7. 0 and below 50℃. When treated at 55 ℃ for ,50 minutes, the activity of recombinant chymosin was 40 percent of the original.
出处
《湖北大学学报（自然科学版）》
CAS
2014年第3期262-266,共5页
Journal of Hubei University：Natural Science
基金
湖北省科技厅公益研究项目(2012DBA28001)
武汉市科技计划项目(2013060501010165)资助
关键词
巴斯德毕赤酵母
牛凝乳酶
基因表达
凝乳酶性质
Pichia pastoris
bovine chymosin
gene expression
characterization of chymosin
